[Experimental studies on urinary incontinence. II. Function and distribution of alpha 1- and alpha 2-adrenoceptors in male and female rabbit urethral smooth muscle].

The effects of various alpha-adrenergic agonists and antagonists on the contractile properties of the isolated urethra of male and female rabbits were examined and the population of alpha 1- and alpha 2-adrenoceptors, using radioligand receptor binding techniques, was also quantified to define the functional role of postsynaptic alpha-adrenoceptors in urethral smooth muscle. Norepinephrine, epinephrine (non-specific alpha-adrenergic agonists) and phenylephrine (alpha 1-adrenergic agonists) induced increases in contractile force in both the male and female urethra. Clonidine (alpha 2-adrenergic agonist) caused a relatively large contractile response in the female urethra but only a small contractile response in the male urethra. Receptor binding studies indicated that the male urethra contains almost equal amounts of alpha 1- and alpha 2-adrenoceptors (32 vs. 34 fmol/mg protein, respectively) whereas the female urethra contains a significantly greater density of alpha 2- than alpha 1-adrenoceptors (122 vs. 34 fmol/mg protein, respectively). Our studies have indicated that: (1) both alpha 1-and alpha 2-adrenoceptors cause contractile responses in male and female rabbit urethra; (2) the greater response to alpha 2-agonist in female than male urethra is correlated with a higher density of alpha 2-adrenoceptors in this tissue.