Su Wenjing, Xia Juan, Chen Xueqin, Xu Miao, Nie Ling, Chen Ni, Gong Jing, Li Xinglan, Zhou Qiao
Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China.
Laboratory of Pathology, State Key Laboratory of Biotherapy and Department of Pathology, West China Hospital, West China Medical School, Sichuan University Chengdu 610041, China ; Suining Central Hospital Suining 629000, China.
Int J Clin Exp Pathol. 2014 Nov 26;7(12):8666-74. eCollection 2014.
The transcriptional factor AP-2α is a tumor suppressor gene and is downregulated in various neoplasms including glioma. Although the level of AP-2α is negatively associated with the grade of human glioma, the specific functions of AP-2α in glioma are still unknown. In this study, we experimentally showed that artificial overexpression of AP-2α in glioma T98G and U251 cells significantly downregulated the mRNA levels of Bcl-xl, Bcl-2, c-IAP2 and survivin, together with upregulation of the Hrk mRNA levels. Reintroduction of AP-2α also induced downregulation of the protein levels of survivin and VEGF in glioma cells. In biological assays with T98G and U251 cells, AP-2α reduced tumor cell growth, increased cell death, attenuated cell migration and endothelial tube formation. The AP-2α transcription factor may play an important role in suppressing glioma progression.
转录因子AP - 2α是一种肿瘤抑制基因，在包括胶质瘤在内的多种肿瘤中表达下调。尽管AP - 2α的水平与人类胶质瘤的分级呈负相关，但其在胶质瘤中的具体功能仍不清楚。在本研究中，我们通过实验表明，在胶质瘤T98G和U251细胞中人工过表达AP - 2α可显著下调Bcl - xl、Bcl - 2、c - IAP2和survivin的mRNA水平，同时上调Hrk的mRNA水平。重新引入AP - 2α也可诱导胶质瘤细胞中survivin和VEGF蛋白水平的下调。在对T98G和U251细胞进行的生物学实验中，AP - 2α可减少肿瘤细胞生长、增加细胞死亡、减弱细胞迁移和内皮管形成。AP - 2α转录因子可能在抑制胶质瘤进展中发挥重要作用。
