Magetsari R
Orthopaedics Department, Sardjito Hospital, Yogyakarta, Indonesia.
Malays Orthop J. 2013 Nov;7(3):10-4. doi: 10.5704/MOJ.1311.010.
Chronic osteomyelitis remains one of the common problems with the use of orthopaedic implants. Staphylococcus epidermidis is notorious for its biofilm formation on indwelling medical devices and is one of the most frequent pathogenic agents in chronic osteomyelitis. Cinnamon oil has been proven to be an effective antimicrobial agent against several bacteria, including S. epidermidis. The eradication of S. epidermidis and prevention of biofilm formation on medical devices are desirable outcomes.
To study the antimicrobial effect of cinnamon oil coating on K-wire against S. epidermidis and to quantify the most effective concentration of cinnamon oil coating on the K-wire.
The cinnamon oil was divided in ten different concentrations, from 0.002% to 1%, and subsequently applied to the Kirschner wire (K-wire). Its antimicrobial effect was determined by agar well diffusion method (MHA). Cinnamon oil coated K-wires were planted on S. epidermidis inoculated Muller-Hinton Agar (MHA) plate. The size of the zone of inhibition was recorded to the nearest mm, and this was compared to gentamycin, fosfomycin, vancomycin, netilmycin.
The cream based 1% concentration cinnamon oil coating on K-wire showed the strongest antimicrobial effect on S. epidermidis inoculated MHA plate. This was evident especially in the fourth repetition, with an inhibition zone diameter (IZD) of 19 mm. In the 1% concentration repetitions, the highest mean IZD of the 4 repetitions was 14 mm (intermediate according NCCLS). The mean IZD results demonstrate that cinnamon oil has 46.3% of the effectiveness of gentamycin, 49.1% of fosfomycin, 59.6% of vancomycin, and 43.4% of netilmycin.
In this in-vitro study, cream based cinnamon oil coating on K-wire is effective against S. epidermidis, though less effective compared to gentamycin, fosfomycin, vancomycin and netilmycin.
Cinnamon oil, K-wire, antimicrobial, S.epidermidis.
慢性骨髓炎仍然是使用骨科植入物时常见的问题之一。表皮葡萄球菌因其在植入式医疗器械上形成生物膜而臭名昭著，是慢性骨髓炎最常见的病原体之一。肉桂油已被证明是一种有效的抗菌剂，可对抗多种细菌，包括表皮葡萄球菌。根除表皮葡萄球菌并防止其在医疗器械上形成生物膜是理想的结果。
研究肉桂油涂层克氏针对表皮葡萄球菌的抗菌作用，并量化肉桂油涂层克氏针的最有效浓度。
将肉桂油分为十种不同浓度，从0.002%到1%，随后应用于克氏针（K针）。通过琼脂孔扩散法（MHA）测定其抗菌效果。将涂有肉桂油的克氏针接种在接种了表皮葡萄球菌的Muller-Hinton琼脂（MHA）平板上。抑制圈大小记录到最接近的毫米，并与庆大霉素、磷霉素、万古霉素、奈替米星进行比较。
基于乳膏的1%浓度肉桂油涂层克氏针对接种了表皮葡萄球菌的MHA平板显示出最强的抗菌作用。这在第四次重复实验中尤为明显，抑菌圈直径（IZD）为19毫米。在1%浓度的重复实验中，4次重复实验的最高平均IZD为14毫米（根据NCCLS为中度）。平均IZD结果表明，肉桂油的有效性分别为庆大霉素的46.3%、磷霉素的49.1%、万古霉素的59.6%和奈替米星的43.4%。
在这项体外研究中，基于乳膏的肉桂油涂层克氏针对表皮葡萄球菌有效，尽管与庆大霉素、磷霉素、万古霉素和奈替米星相比效果较差。
肉桂油；克氏针；抗菌；表皮葡萄球菌
