Magetsari PhD R, Dewo PhD P, Saputro Md B K, Lanodiyu Md Z
Department of Orthopaedic and Traumatology Dr Sardjito General Hospital/Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia.
Malays Orthop J. 2014 Nov;8(3):11-4. doi: 10.5704/MOJ.1411.003.
S. Epidermidis is among the most frequently isolated microorganisms found in -infection related to implanted devices and the formation of biofilm will be more resistant compared to the planktonic form. This study was carried out to determine the effect of coating on stainless steel orthopaedic implants surfaces with cinnamon oil and chitosan as bioadhesive to prevent biofilms formation of S. Epidermidis.The rod shaped stainless steel 316 L orthopaedic implant with 5 mm diameters was coated 2 times using a mixture of cinnamon oil and chitosan 3% and 2% respectively with serial concentration of cinnamon from 0.125% to 2%. The coated implants were then put into tubes that contained bacterial suspension and incubated. Subsequently, the implants were washed with PBS solution followed by MTT soulution and isopropanol acid solution. that related to biofilm formation. The results were expressed in numbers which represents the absorbance level at ELISA readings on 575 nm (A575) wavelength.The stainless steel implant coated with chitosan and cinnamon oil 2% and 1% has lower absorbance level compared with the absorbance level of S.Epidermidis biofilm only. This study showed that mixture of cinnamon oil and chitosan coated on the surface of stainless steel orthopaedic implant has an effect against S.Epidermidis biofilm formation with the minimum cinnamon oil concentration of 1%.
表皮葡萄球菌是植入性装置相关感染中最常分离出的微生物之一，与浮游形式相比，其生物膜的形成具有更强的抗性。本研究旨在确定用肉桂油和壳聚糖作为生物黏附剂对不锈钢骨科植入物表面进行涂层处理，以防止表皮葡萄球菌生物膜形成的效果。将直径为5毫米的棒状316L不锈钢骨科植入物分别用肉桂油和壳聚糖的混合物进行两次涂层处理，壳聚糖浓度为3%，肉桂油浓度依次为0.125%至2%。然后将涂层后的植入物放入含有细菌悬液的试管中进行培养。随后，先用磷酸盐缓冲盐水（PBS）溶液冲洗植入物，再用噻唑蓝（MTT）溶液和异丙醇酸溶液冲洗，这与生物膜形成有关。结果以数字表示，代表酶联免疫吸附测定（ELISA）在575纳米波长下的吸光度水平（A575）。与仅形成表皮葡萄球菌生物膜的吸光度水平相比，涂有2%壳聚糖和1%肉桂油的不锈钢植入物吸光度水平较低。本研究表明，在不锈钢骨科植入物表面涂覆肉桂油和壳聚糖的混合物对表皮葡萄球菌生物膜形成有抑制作用，肉桂油的最低有效浓度为1%。
