Bustamante S A, Forshult M, Lundgren O
Department of Physiology, University of Göteborg, Sweden.
Acta Physiol Scand. 1989 Apr;135(4):469-75. doi: 10.1111/j.1748-1716.1989.tb08605.x.
The migration of epithelial cells along the crypt-villus axis in the small intestine of the rat was followed by labelling epithelial cells during mitosis with [3H]thymidine given i.v. Using two different techniques (autoradiography and determination of tissue radioactivity) it was demonstrated that 6-9 h after giving the tracer the labelled cells had migrated longer in intestinal segments exposed to cholera toxin than in control segments. This effect of cholera toxin was abolished by giving hexamethonium. We have earlier shown that cholera toxin induces fluid secretion to a large extent by activating the enteric nervous system and we conclude from the present observations that cholera toxin in a similar fashion exerts a trophic effect on the intestinal epithelium via intramural nervous reflexes. The importance of co-release of several neurotransmitters in explaining the trophic effect is tentatively discussed.
给大鼠静脉注射[3H]胸腺嘧啶核苷，在有丝分裂期标记上皮细胞，以此追踪大鼠小肠上皮细胞沿隐窝-绒毛轴的迁移。采用两种不同技术（放射自显影和组织放射性测定）表明，给予示踪剂6 - 9小时后，与对照段相比，暴露于霍乱毒素的肠段中标记细胞迁移得更远。六甲铵可消除霍乱毒素的这种作用。我们之前已表明，霍乱毒素通过激活肠神经系统在很大程度上诱导液体分泌，并且从目前的观察结果得出结论，霍乱毒素以类似方式通过壁内神经反射对肠上皮发挥营养作用。初步讨论了几种神经递质共同释放对解释这种营养作用的重要性。
