Banwell J G, Lepot A, Hanke D W, Sigdestad C
Gastroenterology. 1978 Oct;75(4):717-22.
Epithelial renewal of the small intestine was measured in the Syrian hamster utilizing tritiated thymidine by standard autoradiographic and a scintillation counting technique. Scintillation counting of intestinal replicates proved to be as accurate as standard autoradiography. Average mucosal cell turnover was 71 +/- 3.0 hr in jejunum and 79 +/- 4.2 hr in ileum. Scintillation counting was utilized to study the effect of a maximum cholera enterotoxic secretory stimulus on small intestinal mucosal cell turnover. No significant change in epithelial cell migration occurred during cholera enterotoxin (CT)-induced fluid and electrolyte secretion. The rate of decline in radioactivity as a measure of cell turnover in CT-exposed animals was no different from controls. Epithelial cell proliferation 1 to 42 hr after CT exposure showed no difference from controls. Intestinal fluid and electrolyte secretion persisted for 24 hr after CT exposure. It is concluded that (1) the small intestinal epithelial cell migration was unaltered by this metabolic secretory stimulus, and (2) the data are consistent with the concept that epithelial migration after CT exposure was one factor, although not necessarily the major determinant of the progressive decline in intestinal secretory activity.
利用氚标记的胸腺嘧啶核苷，通过标准放射自显影和闪烁计数技术，在叙利亚仓鼠中测量小肠上皮更新情况。结果表明，小肠重复样本的闪烁计数与标准放射自显影一样准确。空肠黏膜细胞平均更新时间为71±3.0小时，回肠为79±4.2小时。利用闪烁计数法研究最大剂量霍乱肠毒素分泌刺激对小肠黏膜细胞更新的影响。在霍乱肠毒素（CT）诱导的液体和电解质分泌过程中，上皮细胞迁移未发生显著变化。以放射性下降速率作为CT暴露动物细胞更新的指标，其与对照组并无差异。CT暴露后1至42小时上皮细胞增殖情况与对照组无差异。CT暴露后肠液和电解质分泌持续24小时。研究得出结论：（1）这种代谢性分泌刺激未改变小肠上皮细胞迁移；（2）这些数据与以下概念一致，即CT暴露后的上皮迁移是一个因素，尽管不一定是肠道分泌活性逐渐下降的主要决定因素。
