Department of Food Engineering, Izmir Institute of Technology, Urla 35437, Izmir, Turkey.
Department of Food Engineering, Izmir Institute of Technology, Urla 35437, Izmir, Turkey.
Int J Food Microbiol. 2015 May 4;200:52-6. doi: 10.1016/j.ijfoodmicro.2015.01.019. Epub 2015 Feb 3.
The present study examined the growth characteristics of Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores after inoculation into apple, pomegranate and pomegranate-apple blend juices (10, 20, 40 and 80%, v/v). Also, the effect of sporulation medium was tested using mineral [Bacillus acidoterrestris agar (BATA) and Bacillus acidocaldarius agar (BAA)] and non-mineral containing media [potato dextrose agar (PDA) and malt extract agar (MEA)]. The juice samples were inoculated separately with approximately 10(5)CFU/mL cells or spores from different sporulation media and then incubated at 37°C for 336 h. The number of cells decreased significantly with increasing pomegranate juice concentration in the blend juices and storage time (p<0.001). Based on the results, 3.17, 3.53, and 3.72 log cell reductions were observed in 40%, 80% blend and pomegranate juices, respectively while the cell counts attained approximately 7.17 log CFU/mL in apple juice after 336 h. On the other hand, the cell growth was inhibited for a certain time, and then the numbers started to increase after 72 and 144 h in 10% and 20% blend juices, respectively. After 336 h, total population among spores produced on PDA, BATA, BAA and MEA indicated 1.49, 1.65, 1.67, and 1.28 log reductions in pomegranate juice; and 1.51, 1.38, 1.40 and 1.16 log reductions in 80% blend juice, respectively. The inhibitory effects of 10%, 20% and 40% blend juices varied depending on the sporulation media used. The results obtained in this study suggested that pomegranate and pomegranate-apple blend juices could inhibit the growth of A. acidoterrestris DSM 3922 vegetative cells and spores.
本研究考察了嗜酸耐热杆菌 DSM 3922 营养细胞和孢子接种到苹果、石榴和石榴苹果混合汁（10%、20%、40%和 80%，v/v）中的生长特性。此外，还测试了使用矿物（嗜酸耐热杆菌琼脂（BATA）和嗜酸芽孢杆菌琼脂（BAA））和非矿物培养基（土豆葡萄糖琼脂（PDA）和麦芽提取物琼脂（MEA））对孢子形成的影响。将来自不同孢子形成培养基的大约 10(5)CFU/mL 细胞或孢子分别接种到果汁样品中，然后在 37°C 下孵育 336 h。随着混合果汁中石榴汁浓度和储存时间的增加（p<0.001），细胞数量显著减少。结果表明，在 40%、80%混合和石榴汁中分别观察到 3.17、3.53 和 3.72 log 细胞减少，而在 336 h 后苹果汁中的细胞计数达到约 7.17 log CFU/mL。另一方面，细胞生长受到一定时间的抑制，然后在 10%和 20%混合果汁中分别在 72 和 144 h 后开始增加。在 336 h 后，在 PDA、BATA、BAA 和 MEA 上产生的孢子总数在石榴汁中分别减少了 1.49、1.65、1.67 和 1.28 log；在 80%混合果汁中分别减少了 1.51、1.38、1.40 和 1.16 log。10%、20%和 40%混合果汁的抑制作用取决于使用的孢子形成培养基。本研究结果表明，石榴和石榴苹果混合汁可以抑制嗜酸耐热杆菌 DSM 3922 营养细胞和孢子的生长。
