吩嗪硫酸甲酯对离体蛙心的细胞毒性作用。

Cytotoxic effect of phenazine methosulphate on the isolated frog heart.

Duncan C J

Department of Zoology, University of Liverpool, UK.

Comp Biochem Physiol A Comp Physiol. 1989;92(1):133-5. doi: 10.1016/0300-9629(89)90755-x.

Abstract

Perfusion of isolated frog hearts with phenazine methosulphate (PMS) at 0.3-1.0 mM caused a fall in amplitude and frequency of beat, and finally a cessation of contractile activity, together with widespread ultrastructural damage. 2. Sarcolemma blebs were a characteristic feature of the damage. 3. No protection was provided by mannitol (10-100 mM), superoxide dismutase, catalase or a pHo of 6.6. 4. Potassium ferricyanide (1-6 mM), an artificial electron acceptor, also caused ultrastructural damage. 5. Comparisons are made with the oxygen paradox of mammalian heart, and the possible role of Ca2+ fluxes and oxygen radicals in muscle damage are discussed.

摘要

用0.3 - 1.0 mM的硫酸吩嗪甲酯（PMS）灌注离体蛙心，会导致心跳幅度和频率下降，最终收缩活动停止，同时伴有广泛的超微结构损伤。2. 肌膜小泡是损伤的一个特征性表现。3. 甘露醇（10 - 100 mM）、超氧化物歧化酶、过氧化氢酶或pH值为6.6均无保护作用。4. 人工电子受体铁氰化钾（1 - 6 mM）也会导致超微结构损伤。5. 将其与哺乳动物心脏的氧反常现象进行了比较，并讨论了Ca2+通量和氧自由基在肌肉损伤中的可能作用。