Kyewski B A, Jenkinson E J, Kingston R, Altevogt P, Owen M J, Owen J J
Institut für Immunologie und Genetik, Deutsches Krebsforschungszentrum, Heidelberg.
Eur J Immunol. 1989 May;19(5):951-4. doi: 10.1002/eji.1830190526.
Using a rat monoclonal antibody against mouse CD2, we determined the expression of this marker on thymocytes during ontogeny. CD2 expression becomes detectable at day 15 and reaches adult levels (approximately 95% positivity) by day 19. Furthermore, the effect of anti-CD2 antibodies on T cell differentiation was analyzed by addition of antibodies to thymus organ cultures or repeated injection into newborn mice. Anti-CD2 antibodies inhibit CD2 expression in organ cultures and drastically reduce its expression on thymocytes and peripheral lymphocytes in vivo. In either situation, suppression of CD2 expression does not significantly alter the generation of T cells expressing CD3, CD4, CD8 and T cell receptor V beta 8. These results do not support a role for CD2 in early steps of thymocyte differentiation.
我们使用一种抗小鼠CD2的大鼠单克隆抗体，确定了该标志物在个体发育过程中胸腺细胞上的表达情况。CD2表达在第15天可检测到，并在第19天达到成年水平（约95%阳性）。此外，通过向胸腺器官培养物中添加抗体或反复注射到新生小鼠体内，分析了抗CD2抗体对T细胞分化的影响。抗CD2抗体在器官培养物中抑制CD2表达，并在体内显著降低其在胸腺细胞和外周淋巴细胞上的表达。在这两种情况下，CD2表达的抑制均未显著改变表达CD3、CD4、CD8和T细胞受体Vβ8的T细胞的生成。这些结果不支持CD2在胸腺细胞分化早期步骤中起作用。
