Sen J, Arceci R J, Jones W, Burakoff S J
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115.
Eur J Immunol. 1989 Jul;19(7):1297-302. doi: 10.1002/eji.1830190722.
CD2 was first defined as the erythrocyte rosetting protein on the surface of human T cells. Recently, the rat and murine homologues have been identified by cDNA cloning. In this report we demonstrate that CD2 is expressed on the surface of most adult murine peripheral lymphocytes and thymocytes by indirect immunofluorescence using an anti-murine CD2 antiserum. The expression of CD2 on murine B cells was unexpected since in rat and human species it has been defined as a T cell-specific marker. Furthermore, CD2 appears very early on fetal thymocytes during development. The level of surface expression increases from day 13 of gestation to day 17, after which the surface density appears to reach a steady state. Thus, CD2 is expressed on day-13 thymocytes at the same stage that Thy-1, Pgp1 and the TcR gamma/delta/CD3 complex have been shown to be expressed.
CD2最初被定义为人T细胞表面的红细胞玫瑰花结形成蛋白。最近，通过cDNA克隆鉴定出了大鼠和小鼠的同源物。在本报告中，我们使用抗小鼠CD2抗血清通过间接免疫荧光证明CD2在大多数成年小鼠外周淋巴细胞和胸腺细胞表面表达。CD2在小鼠B细胞上的表达出乎意料，因为在大鼠和人类中它被定义为T细胞特异性标志物。此外，CD2在发育过程中的胎儿胸腺细胞上很早就出现了。表面表达水平从妊娠第13天到第17天增加，之后表面密度似乎达到稳定状态。因此，CD2在第13天的胸腺细胞上表达，与Thy-1、Pgp1和TcRγ/δ/CD3复合物已被证明表达的阶段相同。
