检测囊性纤维化患者呼吸道中的 KI WU 和 Merkel 细胞多瘤病毒。

Detection of KI WU and Merkel cell polyomavirus in respiratory tract of cystic fibrosis patients.

机构信息

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, Italy.

Cystic Fibrosis Centre, Paediatric Department, Children's Hospital, AO Spedali Civili Brescia, Brescia, Italy.

出版信息

Clin Microbiol Infect. 2015 Jun;21(6):603.e9-15. doi: 10.1016/j.cmi.2015.01.025. Epub 2015 Feb 9.

DOI:10.1016/j.cmi.2015.01.025

PMID:25677628

Abstract

In the last few years, many reports have confirmed the presence of WU, KI and Merkel cell (MC) polyomaviruses (PyV) in respiratory samples wordwide, but their pathogenic role in patients with underlying conditions such as cystic fibrosis is still debated. To determine the prevalence of MCPyV, WUPyV and KIPyV, we conducted a 1-year-long microbiological testing of respiratory specimens from 93 patients with cystic fibrosis in Brescia, Italy. We detected PyV DNA in 94 out of 337 analysed specimens. KIPyV was the most common virus detected (12.1%), followed by WUPyV (8.9%) and MCPyV (6.8%). We found an intriguing association between the presence of MCPyV and the concurrent isolation of Pseudomonas aeruginosa, as well as with the patient status, classified as chronically colonized with P. aeruginosa. Our study adds perspective on the prevalence and the potential pathogenic role of PyV infections.

摘要

在过去的几年中，许多报告已经证实，WU、KI 和 Merkel 细胞（MC）多瘤病毒（PyV）在全球范围内的呼吸道样本中存在，但它们在囊性纤维化等基础疾病患者中的致病作用仍存在争议。为了确定 MCPyV、WUPyV 和 KIPyV 的流行率，我们对来自意大利布雷西亚的 93 名囊性纤维化患者的呼吸道标本进行了为期一年的微生物学检测。我们在 337 个分析标本中的 94 个标本中检测到了 PyV DNA。KIPyV 是最常见的病毒（12.1%），其次是 WUPyV（8.9%）和 MCPyV（6.8%）。我们发现 MCPyV 的存在与铜绿假单胞菌的同时分离以及与患者状况之间存在有趣的关联，患者状况被归类为铜绿假单胞菌的慢性定植。我们的研究增加了 PyV 感染的流行率和潜在致病性作用的视角。