在意大利健康儿童的二级淋巴组织中检测马拉维多瘤病毒序列：一个短暂的感染部位。
Detection of Malawi polyomavirus sequences in secondary lymphoid tissues from Italian healthy children: a transient site of infection.
作者信息
Papa N, Zanotta N, Knowles A, Orzan E, Comar M
机构信息
Institute for Maternal and Child Health - IRCCS "Burlo Garofolo", Via dell'Istria 65, 34137, Trieste, Italy.
Medical Sciences Department, University of Trieste, Piazzale Europa 1, 34128, Trieste, Italy.
出版信息
Virol J. 2016 Jun 10;13:97. doi: 10.1186/s12985-016-0553-z.
BACKGROUND
The novel Malawi polyomavirus (MWPyV) was initially detected in stool specimens from healthy children and children with gastrointestinal symptoms, mostly diarrhea, indicating that MWPyV might play a role in human gastroenteric diseases. Recently, MWPyV sequences were additionally identified in respiratory secretions from both healthy and acutely ill children suggesting that MWPyV may have a tropism for different human tissues. This study was designed to investigate the possible sites of latency/persistence for MWPyV in a cohort of healthy Italian children.
METHODS
Specimens (n° 500) of tonsils, adenoids, blood, urines and feces, from 200 healthy and immunocompetent children (age range: 1-15 years) were tested for the amplification of the MWPyV LT antigen sequence by quantitative real-time PCR. Samples (n° 80) of blood and urines from 40 age-matched children with autoimmune diseases, were screened for comparison. Polyomaviruses JC/BK and Epstein-Barr Virus (EBV) were also tested as markers of infection in all samples using the same molecular technique.
RESULTS
In our series of healthy children, MWPyV was detected only in the lymphoid tissues showing a prevalence of 6 % in tonsils and 1 % in adenoids, although with a low viral load. No JCPyV or BKPyV co-infection was found in MWPyV positive samples, while EBV showed a similar percentage of both in tonsils and adenoids (38 and 37 %). Conversely, no MWPyV DNA was detected in stool from babies with gastroenteric syndrome. With regards to autoimmune children, neither MWPyV nor BKPyV were detected in blood, while JCPyV viremia was observed in 15 % (6/40) of children treated with Infliximab. Urinary BKPyV shedding was observed in 12.5 % (5/40) while JCPyV in 100 % of the samples.
CONCLUSIONS
The detection of MWPyV sequences in tonsils and adenoids of healthy children suggests that secondary lymphoid tissues can harbour MWPyV probably as transient sites of persistence rather than actual sites of latency.
背景
新型马拉维多瘤病毒（MWPyV）最初是在健康儿童和有胃肠道症状（主要是腹泻）的儿童的粪便样本中检测到的，这表明MWPyV可能在人类胃肠疾病中起作用。最近，在健康儿童和急性病儿童的呼吸道分泌物中也发现了MWPyV序列，这表明MWPyV可能对不同的人体组织具有嗜性。本研究旨在调查MWPyV在一组健康意大利儿童中可能的潜伏/持续存在部位。
方法
通过定量实时PCR检测了200名健康且免疫功能正常的儿童（年龄范围：1至15岁）的扁桃体、腺样体、血液、尿液和粪便样本（共500份）中MWPyV大T抗原序列的扩增情况。对40名年龄匹配的患有自身免疫性疾病的儿童的血液和尿液样本（共80份）进行了筛查以作比较。还使用相同的分子技术检测了所有样本中的多瘤病毒JC/BK和爱泼斯坦-巴尔病毒（EBV）作为感染标志物。
结果
在我们的健康儿童系列中，仅在淋巴组织中检测到MWPyV，扁桃体中的患病率为6%，腺样体中的患病率为1%，尽管病毒载量较低。在MWPyV阳性样本中未发现JCPyV或BKPyV合并感染，而EBV在扁桃体和腺样体中的比例相似（分别为38%和37%）。相反，在患有胃肠综合征的婴儿粪便中未检测到MWPyV DNA。关于自身免疫性疾病儿童，在血液中未检测到MWPyV和BKPyV，而在接受英夫利昔单抗治疗的儿童中，15%（6/40）观察到JCPyV病毒血症。在12.5%（5/40）的样本中观察到尿液BKPyV脱落，而JCPyV在100%的样本中被检测到。
结论
在健康儿童的扁桃体和腺样体中检测到MWPyV序列表明，二级淋巴组织可能是MWPyV的藏身之处，可能是短暂的持续存在部位而非真正的潜伏部位。
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