Moynet D, MacLean S J, Ng K H, Anctil D, Gibson D M
J Immunol. 1985 May;134(5):3455-60.
We describe here the cloning and complete sequence analysis of the rearranged kappa variable region gene from the V kappa-1A-producing myeloma (C.AL20-TEPC-105). A 5'-flanking region probe from the V kappa-1A gene has been used to study the V kappa-1 germ-line gene family in strains of mice differing at the Ig kappa-Ef2 locus. All Ef2a strains examined possess an identical pair of BamHI restriction fragments strongly hybridizing to the 5' probe. Surprisingly, only two of the six strains of mice previously designated Ef2b (NZB and C58) possessed clearly altered restriction fragment sizes for V kappa-1 genes. The remaining four Ef2b strains, namely BDP/J, CE/J, I/LnJ, and P/J, appear to carry V kappa-1 genes similar to those of Ef2a strains. It is suggested that these strains may carry a third form of the V kappa-1A gene, differing in the protein coding region but indistinguishable at the DNA level with the use of BamHI or EcoRI. Alternatively, these strains may fail to express V kappa-1A light chains due to a regulatory defect involving this subgroup of kappa genes.
我们在此描述了来自产生Vκ-1A的骨髓瘤（C.AL20-TEPC-105）的重排κ可变区基因的克隆及完整序列分析。已使用来自Vκ-1A基因的5'侧翼区探针，研究在Igκ-Ef2基因座存在差异的小鼠品系中的Vκ-1种系基因家族。所有检测的Ef2a品系都拥有一对与5'探针强烈杂交的相同BamHI限制性片段。令人惊讶的是，先前指定为Ef2b的六个小鼠品系中只有两个（NZB和C58）的Vκ-1基因的限制性片段大小明显改变。其余四个Ef2b品系，即BDP/J、CE/J、I/LnJ和P/J，似乎携带与Ef2a品系相似的Vκ-1基因。有人提出，这些品系可能携带Vκ-1A基因的第三种形式，在蛋白质编码区有所不同，但在DNA水平上使用BamHI或EcoRI无法区分。或者，这些品系可能由于涉及该κ基因亚组的调节缺陷而无法表达Vκ-1A轻链。
