Armet是一种介导蚜虫与植物相互作用的效应蛋白。
Armet is an effector protein mediating aphid-plant interactions.
作者信息
Wang Wei, Dai Huaien, Zhang Yi, Chandrasekar Raman, Luo Lan, Hiromasa Yasuaki, Sheng Changzhong, Peng Gongxin, Chen Shaoliang, Tomich John M, Reese John, Edwards Owain, Kang Le, Reeck Gerald, Cui Feng
机构信息
*State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia.
*State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas, USA; Department of Mathematics, Hebei University of Science and Technology/Hebei Laboratory of Pharmaceutic Molecular Chemistry, Shijiazhuang, Hebei, China; Department of Entomology, Kansas State University, Manhattan, Kansas, USA; and Commonwealth Scientific and Industrial Research Organisation Ecosystem Sciences, Centre for Environment and Life Sciences, Floreat, Australia
出版信息
FASEB J. 2015 May;29(5):2032-45. doi: 10.1096/fj.14-266023. Epub 2015 Feb 12.
Aphid saliva is predicted to contain proteins that modulate plant defenses and facilitate feeding. Armet is a well-characterized bifunctional protein in mammalian systems. Here we report a new role of Armet, namely as an effector protein in the pea aphid, Acyrthosiphon pisum. Pea aphid Armet's physical and chemical properties and its intracellular role are comparable to those reported for mammalian Armets. Uniquely, we detected Armet in aphid watery saliva and in the phloem sap of fava beans fed on by aphids. Armet's transcript level is several times higher in the salivary gland when aphids feed on bean plants than when they feed on an artificial diet. Knockdown of the Armet transcript by RNA interference disturbs aphid feeding behavior on fava beans measured by the electrical penetration graph technique and leads to a shortened life span. Inoculation of pea aphid Armet protein into tobacco leaves induced a transcriptional response that included pathogen-responsive genes. The data suggest that Armet is an effector protein mediating aphid-plant interactions.
预计蚜虫唾液中含有能够调节植物防御并便于取食的蛋白质。Armet是哺乳动物系统中一种特性明确的双功能蛋白。在此我们报道Armet的一个新作用，即在豌豆蚜（Acyrthosiphon pisum）中作为一种效应蛋白。豌豆蚜Armet的物理和化学性质及其在细胞内的作用与已报道的哺乳动物Armet的性质和作用相当。独特的是，我们在蚜虫的水样唾液以及蚜虫取食的蚕豆韧皮部汁液中检测到了Armet。当蚜虫取食豆类植物时，Armet在唾液腺中的转录水平比取食人工饲料时高出几倍。通过RNA干扰敲低Armet转录本会干扰用电穿透图技术测定的蚜虫在蚕豆上的取食行为，并导致寿命缩短。将豌豆蚜Armet蛋白接种到烟草叶片中会诱导包括病原体响应基因在内的转录反应。数据表明Armet是介导蚜虫与植物相互作用的效应蛋白。
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