Xue Hui, Yan Mengjie, Zhu Xiangzhen, Wang Li, Chen Lizhen, Luo Junyu, Cui Jinjie, Gao Xueke
Research Base of Zhengzhou University, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Front Plant Sci. 2024 Nov 28;15:1500834. doi: 10.3389/fpls.2024.1500834. eCollection 2024.
Aphids are insects that feed on phloem and introduce effector proteins into plant cells through saliva. These effector proteins are key in regulating host plant defense and enhancing aphid host adaptation. We identified these salivary proteins in the cotton aphids genome and named them AgoArmet and AgoC002. Multiple sequence alignment, protein structure analysis, and phylogenetic analysis of these proteins with related proteins from other insects showed that AgoArmet and Armet of have high sequence identity (97%) and belong to the same evolutionary branch and that AgoC002 shares the highest sequence identity (80%) and closest evolutionary relationship with C002 of . Expression profiling of AgoArmet and AgoC002 showed that they were most highly expressed in cotton aphids during the adult-3d period. Cotton aphids transferred to zucchini leaves resulted in a significant increase in the expression of and within 48h. To investigate the functions of and , we decreased the expression of these genes in cotton using virus-induced gene silencing (VIGS), which ultimately led to a 38% and 26% decrease in cotton aphids fecundity, respectively. Moreover, the reduction in expression resulted in a significant (24%) reduction in body weight. Taken together, our findings demonstrate that AgoArmet and AgoC002 are key effector proteins involved in cotton aphids feeding and host adaptation.
蚜虫是以韧皮部为食并通过唾液将效应蛋白导入植物细胞的昆虫。这些效应蛋白在调节寄主植物防御和增强蚜虫寄主适应性方面起着关键作用。我们在棉蚜基因组中鉴定出这些唾液蛋白，并将它们命名为AgoArmet和AgoC002。对这些蛋白质与其他昆虫相关蛋白质进行多序列比对、蛋白质结构分析和系统发育分析表明，AgoArmet与[某昆虫]的Armet具有较高的序列同一性（97%），属于同一进化分支，且AgoC002与[某昆虫]的C002具有最高的序列同一性（80%）和最密切的进化关系。AgoArmet和AgoC002的表达谱分析表明，它们在成年后3天的棉蚜中表达量最高。转移到西葫芦叶片上的棉蚜在48小时内导致[相关基因]的表达显著增加。为了研究[相关基因]的功能，我们利用病毒诱导基因沉默（VIGS）降低棉花中这些基因的表达，最终分别导致棉蚜繁殖力下降38%和26%。此外，[相关基因]表达的降低导致体重显著下降（24%）。综上所述，我们的研究结果表明，AgoArmet和AgoC002是参与棉蚜取食和寄主适应性的关键效应蛋白。
