Gebrekidan Atsbha Gebreslasie, Zhang Yong, Chen Julian
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100875, China.
Curr Issues Mol Biol. 2024 Nov 23;46(12):13383-13404. doi: 10.3390/cimb46120798.
The Rose grain aphid, a notable agricultural pest, releases saliva while feeding. Yet, there is a need for a comprehensive understanding of the specific identity and role of secretory proteins released during probing and feeding. Therefore, a combined transcriptomic and proteomic approach was employed in this study to identify putative secretory proteins. The transcriptomic sequencing result led to the assembly of 18,030 unigenes out of 31,344 transcripts. Among these, 705 potential secretory proteins were predicted and functionally annotated against publicly accessible protein databases. Notably, a substantial proportion of secretory genes (71.5%, 69.08%, and 60.85%) were predicted to encode known proteins in Nr, Pfam, and Swiss-Prot databases, respectively. Conversely, 27.37% and 0.99% of gene transcripts were predicted to encode known proteins with unspecified functions in the Nr and Swiss-Prot databases, respectively. Meanwhile, the proteomic analysis result identified, 15 salivary proteins. Interestingly, most salivary proteins (i.e., 60% of the proteins) showed close similarity to , while 46.67% showed close similarity to , and . However, to verify the expression of these secretory genes and characterize the biological function of salivary proteins further investigation should be geared towards gene expression and functional analysis.
蔷薇长管蚜是一种著名的农业害虫，在取食时会分泌唾液。然而，对于其在刺探和取食过程中分泌的蛋白质的具体特性和作用，仍需要全面了解。因此，本研究采用转录组学和蛋白质组学相结合的方法来鉴定假定的分泌蛋白。转录组测序结果从31,344条转录本中组装得到18,030个单基因。其中，预测了705个潜在的分泌蛋白，并针对可公开访问的蛋白质数据库进行了功能注释。值得注意的是，分别有相当比例的分泌基因（71.5%、69.08%和60.85%）在Nr、Pfam和Swiss-Prot数据库中被预测编码已知蛋白。相反，分别有27.37%和0.99%的基因转录本在Nr和Swiss-Prot数据库中被预测编码功能未明确的已知蛋白。同时，蛋白质组学分析结果鉴定出15种唾液蛋白。有趣的是，大多数唾液蛋白（即60%的蛋白）与 显示出密切相似性，而46.67%与 、 和 显示出密切相似性。然而，为了验证这些分泌基因的表达并进一步表征唾液蛋白的生物学功能，应针对基因表达和功能分析展开进一步研究。
