携带β-环糊精的二茂铁基萘二亚胺的协同结合将双链DNA转化为棒状结构。

Cooperative binding of ferrocenylnaphthalene diimide carrying β-cyclodextrin converts double-stranded DNA to a rod-like structure.

作者信息

Sato Shinobu, Umeda Yuta, Fujii Satoshi, Takenaka Shigeori

机构信息

†Department of Applied Chemistry, ‡Research Center for Biomicrosensing Technology, and §Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka 804-8550, Japan.

出版信息

Bioconjug Chem. 2015 Mar 18;26(3):379-82. doi: 10.1021/bc500535n. Epub 2015 Feb 20.

DOI:10.1021/bc500535n

PMID:25679261

Abstract

Ferrocenylnaphthalene diimide carrying β-cyclodextrin (β-CD), 1, intercalated into double-stranded DNA with a binding affinity of K = (6.6 ± 0.8) × 10(4) M(-1) and a binding site size of n = 4, with a high positive cooperative parameter of ω = 14. β-CD and ferrocene moieties of the compound contributed to the formation of the intermolecular inclusion complex on DNA. Binding of 1 resulted in conversion of the DNA duplex to a rod-like form, which was cleaved upon adamantylamine addition.

摘要

携带β-环糊精（β-CD）的二茂铁基萘二酰亚胺1，以K = (6.6 ± 0.8) × 10⁴ M⁻¹的结合亲和力和n = 4的结合位点大小插入双链DNA中，具有ω = 14的高正协同参数。该化合物的β-环糊精和二茂铁部分有助于在DNA上形成分子间包合物。1的结合导致DNA双链体转变为棒状形式，在加入金刚烷胺后会被切割。