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对囊性纤维化患者铜绿假单胞菌碳青霉烯酶产生情况的表型筛选试验评估

Evaluation of phenotypic screening tests for carbapenemase production in Pseudomonas aeruginosa from patients with cystic fibrosis.

作者信息

Tai Anna S, Sidjabat Hanna E, Kidd Timothy J, Whiley David M, Paterson David L, Bell Scott C

机构信息

Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia; Adult Cystic Fibrosis Centre, Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia; School of Medicine, The University of Queensland, Brisbane, Australia.

University of Queensland, UQ Centre for Clinical Research (UQCCR), Brisbane, Australia.

出版信息

J Microbiol Methods. 2015 Apr;111:105-7. doi: 10.1016/j.mimet.2015.02.006. Epub 2015 Feb 10.

DOI:10.1016/j.mimet.2015.02.006
PMID:25680305
Abstract

Carbapenemase phenotypic tests (inhibitor-based, modified Hodge and Carba NP) for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF) were evaluated with PCR as a reference assay. Except for Carba NP, all methods demonstrated high false positives. Carba NP test presents a promising screening method for CF P. aeruginosa isolates.

摘要

以聚合酶链反应(PCR)作为参考检测方法,对来自囊性纤维化(CF)患者的铜绿假单胞菌分离株进行碳青霉烯酶表型检测(基于抑制剂的检测、改良Hodge试验和Carba NP试验)。除Carba NP试验外,所有方法均显示出高假阳性率。Carba NP试验是一种用于CF铜绿假单胞菌分离株的有前景的筛查方法。

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