Gutiérrez Pablo A, Alzate Juan F, Montoya Mauricio Marín
Laboratorio de Microbiología Industrial, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59A No 63 - 20, Medellín, Colombia,
Virus Genes. 2015 Jun;50(3):518-22. doi: 10.1007/s11262-015-1181-1. Epub 2015 Feb 14.
Transcriptome analysis of a Cape gooseberry (Physalis peruviana) plant with leaf symptoms of a mild yellow mosaic typical of a viral disease revealed an infection with Potato virus X (PVX). The genome sequence of the PVX-Physalis isolate comprises 6435 nt and exhibits higher sequence similarity to members of the Eurasian group of PVX (95 %) than to the American group (77 %). Genome organization is similar to other PVX isolates with five open reading frames coding for proteins RdRp, TGBp1, TGBp2, TGBp3, and CP. 5' and 3' untranslated regions revealed all regulatory motifs typically found in PVX isolates. The PVX-Physalis genome is the only complete sequence available for a Potexvirus in Colombia and is a new addition to the restricted number of available sequences of PVX isolates infecting plant species different to potato.
对一株灯笼果（酸浆属秘鲁酸浆）进行转录组分析，该植株叶片出现典型病毒病的轻度黄色花叶症状，结果显示其感染了马铃薯X病毒（PVX）。PVX-酸浆分离株的基因组序列由6435个核苷酸组成，与欧亚组PVX成员的序列相似性更高（约95%），而与美洲组的相似性较低（约77%）。基因组结构与其他PVX分离株相似，有五个开放阅读框，分别编码RdRp、TGBp1、TGBp2、TGBp3和CP蛋白。5'和3'非翻译区显示出PVX分离株中常见的所有调控基序。PVX-酸浆基因组是哥伦比亚可获得的唯一完整的马铃薯X病毒属序列，也是感染非马铃薯植物物种的PVX分离株有限可用序列中的新增序列。
