Dey Priyankar, Chaudhuri Tapas Kumar
Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, 734013, West Bengal, India.
Cytotechnology. 2016 Aug;68(4):749-61. doi: 10.1007/s10616-014-9826-9. Epub 2015 Feb 14.
Nerium indicum is a medicinal plant which is used in the treatment of wide range of illness in different ethnopharmacological practices. We have studied the immunomodulatory activity of the 70 % hydro-methanolic extract of N. indicum leaves (NILE) by studying plaque forming cell assay, haemagglutination titre, cell proliferation assay, inhibition of nitric oxide expression and cyclooxygenase activity, estimation of IL-2, IFN-γ, IL-4, IL-10, TNF-α and IgM levels. Furthermore, we have characterized NILE with Fourier Transform Infra-red (FTIR) spectroscopy. The results displayed that NILE possessed potent immunomodulatory activity by up-regulating IL-2, IFN-γ, IL-10 expression and down-regulating IL-4, TNF-α expression in vitro. NILE also stimulated the in vivo expression of immunoglobulin level in mouse. FTIR analysis revealed phytocompounds of diverse chemical nature present in NILE. Thus, the potent immunomodulatory activity of N. indicum may have serious implications in the treatment of inflammatory diseases in the future.
夹竹桃是一种药用植物，在不同的民族药理学实践中用于治疗多种疾病。我们通过研究空斑形成细胞试验、血凝滴度、细胞增殖试验、一氧化氮表达抑制和环氧化酶活性、白细胞介素-2、干扰素-γ、白细胞介素-4、白细胞介素-10、肿瘤坏死因子-α和免疫球蛋白M水平的测定，研究了夹竹桃叶70%甲醇提取物（NILE）的免疫调节活性。此外，我们用傅里叶变换红外（FTIR）光谱对NILE进行了表征。结果显示，NILE在体外通过上调白细胞介素-2、干扰素-γ、白细胞介素-10的表达和下调白细胞介素-4、肿瘤坏死因子-α的表达而具有强大的免疫调节活性。NILE还刺激了小鼠体内免疫球蛋白水平的表达。FTIR分析揭示了NILE中存在多种化学性质的植物化合物。因此，夹竹桃的强大免疫调节活性可能在未来炎症性疾病的治疗中具有重要意义。
