Mae S H W, Sofia M, Bolhuis R L H, Nooter K, Oostrum R G, Subagus W, Ibnu G G
Faculty of Medicine, Gadjah Mada University, Yogyakarta.
Med J Malaysia. 2008 Jul;63 Suppl A:24-5.
The leaves of Nerium indicum Mill. have been utilized traditionally to cure cancer. By Bioassay (BST) guided isolation method, six compounds were isolated from the CHCl3 extract of the leaves. Selectivity of these compounds (in 0.6-12,500 ng/ml) was tested on various human cancer (MCF7, EVSA-T, T47D, H226, IGROV, A498, WIDR, M19, HeLa) and normal (Vero) cells in vitro. Doxorubicin and cysplatin were used as positive controls. The result indicated that NiO2D (5alpha-oleandrin) possessed the best cytotoxic effect on HeLa cells (IC50, 8.38 x10(-6) mM) and NiO2C (16, 17-dehidrodeasetil-5alpha-oleandrin) on A498 cells (IC50, 1.43 x 10(-6) mM). Those two compounds were not cytotoxic to normal cell.
夹竹桃叶传统上被用于治疗癌症。通过生物测定（BST）引导的分离方法，从夹竹桃叶的氯仿提取物中分离出六种化合物。测试了这些化合物（浓度为0.6 - 12500 ng/ml）对多种人类癌细胞（MCF7、EVSA - T、T47D、H226、IGROV、A498、WIDR、M19、HeLa）和正常细胞（Vero）的体外选择性。阿霉素和顺铂用作阳性对照。结果表明，NiO2D（5α - 夹竹桃苷元）对HeLa细胞具有最佳的细胞毒性作用（IC50，8.38×10(-6) mM），而NiO2C（16,17 - 去氢乙酰基 - 5α - 夹竹桃苷元）对A498细胞具有最佳细胞毒性作用（IC50，1.43×10(-6) mM）。这两种化合物对正常细胞无细胞毒性。
