Chandra S, Prasad A, Yadav N, Latchumikanthan A, Rakesh R L, Praveen K, Khobra V, Subramani K V, Misri J, Sankar M
Network Programme on Gastrointestinal Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243 122, India.
Network Programme on Gastrointestinal Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar-243 122, India.
Vet Parasitol. 2015 Mar 15;208(3-4):263-7. doi: 10.1016/j.vetpar.2015.01.005. Epub 2015 Jan 17.
Present study was designed to survey the status of benzimidazole (BZ) resistance in goat flocks of different agro climatic regions of Uttar Pradesh (U.P.), India by faecal egg count reduction test (FECRT) and allele specific PCR (AS-PCR). Faecal egg counts and larval culture were made from representative samples of all regions. The results of the faecal culture and PCR-RFLP on beta tubulin isotype-1 gene showed Haemonchus contortus was predominant species. FECRT results showed BZ resistance prevalent in all regions. However, six farms of twenty screened, harboured susceptible populations of strongyles. Three hundred H. contortus infective larvae from all regions of the state were genotyped for BZ resistance. AS-PCR results revealed 55-85% of H. contortus homozygous resistant (rr), 10-21% homozygous susceptible (SS) and 5-24% heterozygous (rS) among different regions of U.P. The allele frequencies were 67-87.5% for resistant (TTC) and 12.5-33% for susceptible (TAC). The survey indicated that the status of BZ resistance is in alarming conditions in all the parts of the state.
本研究旨在通过粪便虫卵计数减少试验（FECRT）和等位基因特异性PCR（AS-PCR），调查印度北方邦不同农业气候区山羊群中苯并咪唑（BZ）的耐药状况。对所有地区的代表性样本进行粪便虫卵计数和幼虫培养。粪便培养和β-微管蛋白同型-1基因的PCR-RFLP结果显示，捻转血矛线虫是主要种类。FECRT结果显示，所有地区都存在BZ耐药情况。然而，在筛选的20个养殖场中，有6个养殖场的圆线虫种群易感。对该邦所有地区的300条捻转血矛线虫感染性幼虫进行了BZ耐药基因分型。AS-PCR结果显示，在北方邦不同地区，55%-85%的捻转血矛线虫为纯合抗性（rr），10%-21%为纯合易感（SS），5%-24%为杂合（rS）。抗性（TTC）等位基因频率为67%-87.5%，易感（TAC）等位基因频率为12.5%-33%。调查表明，该邦所有地区的BZ耐药状况令人担忧。
