Manzano-Moreno Francisco J, Herrera-Briones Francisco J, Linares-Recatala Macarena, Ocaña-Peinado Francisco M, Reyes-Botella Candela, Vallecillo-Capilla Manuel F
Assistant Professor, Department of Oral and Maxillofacial Surgery, University of Granada, Granada, Spain.
Assistant Professor, Department of Oral and Maxillofacial Surgery, University of Granada, Granada, Spain.
J Oral Maxillofac Surg. 2015 Mar;73(3):424-9. doi: 10.1016/j.joms.2014.10.020. Epub 2014 Oct 30.
The aim of this study was to compare levels of bacterial contamination of autogenous bone collected when using low-speed drilling, a back-action chisel, and a bone filter.
Bone tissue samples were taken from 31 patients who underwent surgical extraction of their third lower molars. Before surgical removal of the molar, bone particles were collected by a low-speed drill or a back-action chisel. Then, a stringent aspiration protocol was applied during the ostectomy to collect particulate bone by a bone filter. Processing of samples commenced immediately by incubation in an anaerobic or a CO2-rich atmosphere. The number of colony-forming units (CFUs) was determined at 48 hours of culture.
No significant difference in the number of CFUs per milliliter was observed between the low-speed drilling group and the back-action chisel group in the anaerobic or CO2-rich condition (P = .34). However, significantly more micro-organisms were found in the bone filter group than in the low-speed drilling group or the back-action chisel group in the anaerobic and CO2-rich conditions (P < .001).
Particulate bone harvested with low-speed drilling or a back-action chisel is safer for use as an autograft than are bone particles collected with a bone filter. These results suggest that bone obtained from low-speed drilling is safe and straightforward to harvest and could be the method of choice for collecting particulate bone. Further research is needed to lower the bacterial contamination levels of autogenous bone particles used as graft material.
本研究旨在比较使用低速钻、反作用凿和骨过滤器采集的自体骨的细菌污染水平。
从31例接受下颌第三磨牙手术拔除的患者身上采集骨组织样本。在手术拔除磨牙前，用低速钻或反作用凿收集骨颗粒。然后，在骨切除术期间采用严格的抽吸方案，用骨过滤器收集颗粒状骨。样本采集后立即在厌氧或富二氧化碳环境中培养。在培养48小时后测定菌落形成单位（CFU）的数量。
在厌氧或富二氧化碳条件下，低速钻组和反作用凿组每毫升CFU数量无显著差异（P = 0.34）。然而，在厌氧和富二氧化碳条件下，骨过滤器组中发现的微生物明显多于低速钻组或反作用凿组（P < 0.001）。
与用骨过滤器收集的骨颗粒相比，用低速钻或反作用凿采集的颗粒状骨作为自体骨移植更安全。这些结果表明，通过低速钻获得的骨采集安全且简便，可能是收集颗粒状骨的首选方法。需要进一步研究以降低用作移植材料的自体骨颗粒的细菌污染水平。
