Hashemi Hamid Mahmood, Beshkar Majid
Shariati Hospital, Tehran University of Medical Sciences, Kargar St., Tehran, Iran.
Br J Oral Maxillofac Surg. 2011 Sep;49(6):474-7. doi: 10.1016/j.bjoms.2010.08.003. Epub 2010 Sep 9.
The aim was to compare the degree of microbial contamination of autogenous bone collected by a bone filter with that of autogenous bone harvested by a rongeur during implant surgery. Thirty healthy patients had dental implants inserted. A strict aspiration protocol was used during the operation to collect particulate bone with minimal risk of contamination by oral flora. A fragment of bone (mainly from the tuberosity) was also harvested with a rongeur. Samples from both groups were sent to the laboratory for the microbes to be counted. All samples yielded viable micro-organisms. There was no significant difference between the number of aerobes in the bone filter and those in the bone fragment group (p=0.9). However, there were significantly more anaerobes in the bone filter group than in the bone fragment group. There were significantly more micro-organisms (both aerobes and anaerobes) in the bone filter group than the bone fragment group (p=0.0001). Even with the use of a stringent aspiration protocol the degree of bacterial contamination was significantly higher in collected bone debris than in bone harvested by rongeur during implant surgery.
目的是比较在种植手术中，使用骨过滤器收集的自体骨与使用咬骨钳获取的自体骨的微生物污染程度。30名健康患者接受了牙种植体植入。手术过程中采用严格的抽吸方案，以收集受口腔菌群污染风险最小的颗粒状骨。还用咬骨钳获取了一块骨碎片（主要来自结节）。两组样本均送至实验室进行微生物计数。所有样本均培养出活的微生物。骨过滤器组中的需氧菌数量与骨碎片组中的需氧菌数量之间无显著差异（p = 0.9）。然而，骨过滤器组中的厌氧菌数量明显多于骨碎片组。骨过滤器组中的微生物（需氧菌和厌氧菌）数量明显多于骨碎片组（p = 0.0001）。即使采用严格的抽吸方案，在种植手术中，收集的骨碎片中的细菌污染程度仍明显高于用咬骨钳获取的骨中的细菌污染程度。
