Department of Dental Implant Center, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.
Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
Oral Dis. 2017 Nov;23(8):1099-1108. doi: 10.1111/odi.12704. Epub 2017 Jul 21.
The aim was to compare the osteoblast activity and osteogenic potential of autogenous bone particles harvested using three different techniques and determine the most advantageous method of collecting autogenous bone particles.
Bone particles were harvested from 20 patients during dental implant surgery using bone scraping, low-speed drilling and bone trap filtering. After the osteoblasts were cultured, cell proliferation, migration, mineralization, transcription of osteogenesis-related genes, secretion of osteogenesis-related proteins and osteoinductive protein content in the bone particle matrix were evaluated.
Osteoblast activity and osteogenic potential were higher in bone samples harvested by scraper or low-speed drilling than in samples harvested by bone trap filter. Although these parameters were slightly lower in the low-speed drilling group than in the scraper group, significant differences were found only in bone Gla protein levels. However, the levels of osteoinductive proteins in the bone particle matrix were significantly higher in the low-speed drilling group than in the scraper group.
Low-speed drilling is a recommendable and effective technique for collecting autogenous bone particles. In implant operations, low-speed drilling can be considered the first-line option, and if the quantity of harvested bone is insufficient, bone shavings obtained by the scraper may be considered.
比较三种不同技术采集的自体骨颗粒的成骨细胞活性和成骨潜能，确定采集自体骨颗粒最有利的方法。
在 20 例牙种植术中，使用骨刮除、低速钻取和骨滤器过滤三种方法从患者中获取骨颗粒。培养成骨细胞后，评估骨颗粒基质中成骨相关基因转录、成骨相关蛋白分泌、矿化及细胞增殖、迁移情况。
与骨滤器组相比，刮除和低速钻取组的成骨细胞活性和成骨潜能更高。低速钻取组虽然各项参数略低于刮除组，但仅骨 Gla 蛋白水平有显著差异。然而，低速钻取组骨颗粒基质中的诱导蛋白水平明显高于刮除组。
低速钻取是一种可取且有效的自体骨颗粒采集技术。在种植手术中，低速钻取可作为首选方法，如果采集的骨量不足，可以考虑使用刮勺获取骨屑。
