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重新思考微小RNA与信使核糖核酸的相互作用：相互交织的问题困扰着靶标发现。

Re-thinking miRNA-mRNA interactions: intertwining issues confound target discovery.

作者信息

Cloonan Nicole

机构信息

QIMR Berghofer Medical Research Institute, Genomic Biology Lab, Herston, QLD, Australia.

出版信息

Bioessays. 2015 Apr;37(4):379-88. doi: 10.1002/bies.201400191. Epub 2015 Feb 12.

DOI:10.1002/bies.201400191
PMID:25683051
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4671252/
Abstract

Despite a library full of literature on miRNA biology, core issues relating to miRNA target detection, biological effect, and mode of action remain controversial. This essay proposes that the predominant mechanism of direct miRNA action is translational inhibition, whereas the bulk of miRNA effects are mRNA based. It explores several issues confounding miRNA target detection, and discusses their impact on the dominance of "miRNA seed" dogma and the exploration of non-canonical binding sites. Finally, it makes comparisons between miRNA target prediction and transcription factor binding prediction, and questions the value of characterizing miRNA binding sites based on which miRNA nucleotides are paired with an mRNA.

摘要

尽管有大量关于微小RNA（miRNA）生物学的文献，但与miRNA靶标检测、生物学效应及作用模式相关的核心问题仍存在争议。本文提出，miRNA直接作用的主要机制是翻译抑制，而miRNA的大部分效应基于信使核糖核酸（mRNA）。本文探讨了几个混淆miRNA靶标检测的问题，并讨论了它们对“miRNA种子”法则主导地位及非经典结合位点探索的影响。最后，本文对miRNA靶标预测和转录因子结合预测进行了比较，并质疑基于哪些miRNA核苷酸与mRNA配对来表征miRNA结合位点的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/beb314c6a0e8/bies0037-0379-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/d3eca9eea0f3/bies0037-0379-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/69b71e49501e/bies0037-0379-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/beb314c6a0e8/bies0037-0379-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/d3eca9eea0f3/bies0037-0379-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/69b71e49501e/bies0037-0379-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eacf/4671252/beb314c6a0e8/bies0037-0379-f3.jpg

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Spurious transcription factor binding: non-functional or genetically redundant?假性转录因子结合：无功能还是基因冗余？
High expression of miR-7974 predicts poor prognosis and is associated with autophagy in estrogen receptor-positive breast cancer.
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