Krallmann-Wenzel U, Ott M, Hacker J, Schmidt G
Institut für Experimentelle Biologie und Medizin, Borstel, F.R.G.
FEMS Microbiol Lett. 1989 Apr;49(2-3):315-21. doi: 10.1016/0378-1097(89)90060-8.
DNA hybridization experiments demonstrated that the gene clusters encoding the F8 fimbriae (fei) as well as the type I fimbriae (pil) exist in a single copy on the chromosome of E. coli O18:K5 strain 2980. In conjugation experiments with appropriate donors, the chromosomal site of these gene clusters was determined. The pil genes were mapped close to the gene clusters thr and leu controlling the biosynthesis of threonine and leucine, respectively. The fei genes were found to be located close to the galactose operon (gal) between the position 17 and 21 of the E. coli chromosomal linkage map.
DNA杂交实验表明，编码F8菌毛（fei）以及I型菌毛（pil）的基因簇在大肠杆菌O18:K5菌株2980的染色体上以单拷贝形式存在。在与合适供体的接合实验中，确定了这些基因簇在染色体上的位置。pil基因被定位在分别控制苏氨酸和亮氨酸生物合成的基因簇thr和leu附近。发现fei基因位于大肠杆菌染色体连锁图谱第17至21位之间靠近半乳糖操纵子（gal）的位置。
