Buchanan K, Falkow S, Hull R A, Hull S I
J Bacteriol. 1985 May;162(2):799-803. doi: 10.1128/jb.162.2.799-803.1985.
Type 1 pili, characterized by mannose-inhibitable agglutination of fowl or guinea pig erythrocytes, have been found throughout the family Enterobacteriaceae. A radiolabeled probe was prepared from a restriction endonuclease-digested fragment of the Escherichia coli pil operon and used to detect homologous DNA sequences in 236 bacteria representing 11 genera of Enterobacteriaceae. Only isolates identified as E. coli or Shigella spp. exhibited homology. In contrast, mannose-sensitive hemagglutination was observed in nine genera. Probe DNA did not hybridize to plasmid DNA, indicating a chromosomal location for the pil operon. Analysis of restriction nuclease-digested whole-cell DNA from 60 E. coli and two Shigella sp. isolates indicated that internal sequences were conserved in most strains, but that changes in flanking sequences in the chromosome were common.
1型菌毛的特征是能使鸡或豚鼠红细胞发生甘露糖抑制性凝集，已在整个肠杆菌科中发现。从大肠杆菌菌毛操纵子的限制性内切酶消化片段制备了放射性标记探针，并用于检测代表肠杆菌科11个属的236种细菌中的同源DNA序列。只有鉴定为大肠杆菌或志贺氏菌属的分离株表现出同源性。相比之下，在9个属中观察到了甘露糖敏感的血凝反应。探针DNA未与质粒DNA杂交，表明菌毛操纵子位于染色体上。对60株大肠杆菌和2株志贺氏菌分离株的限制性核酸酶消化全细胞DNA分析表明，大多数菌株的内部序列是保守的，但染色体侧翼序列的变化很常见。
