Chang Yan-Zin, Lin Hsiao-Chun, Chan Shu-Ting, Yeh Shu-Lan
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC.
Department of Nutritional Science, Chung Shan Medical University, Taichung, Taiwan, ROC.
Food Chem. 2012 Jul 15;133(2):445-50. doi: 10.1016/j.foodchem.2012.01.060. Epub 2012 Jan 27.
A549 cells were pre-incubated with β-carotene (BC) alone or in combination with quercetin or three major quercetin metabolites in human plasma, quercetin 3-glucuronide (Q3G), quercetin 3'-sulphate (Q3'S) and isorhamnetin, followed by incubation with benzo[a]pyrene (BaP), to investigate the effects of these compounds on the BaP-induced harmful effects of BC. All the quercetin metabolites at 10μM inhibited BaP+BC-induced cell death. Q3'S, Q3G and isorhamnetin also significantly decreased BaP±BC-induced DNA damage by 64%, 60% and 24%, respectively. In a similar order, these compounds suppressed BaP+BC-induced cytochrome P450 (CYP)1A1/1A2 expression by 10-50%. Q3G and Q3'S significantly decreased the intracellular reactive oxygen species formation induced by BaP+BC; however, Q3G had the best effect on decreasing the loss of BC induced by Fe/NTA. The combined effects of quercetin metabolites were additive. This study indicates that quercetin metabolites decrease the BaP-induced harmful effect of β-carotene in A549 cells by downregulating the expression of CYP1A1/1A2, at least in part.
将A549细胞单独用β-胡萝卜素（BC）预处理，或与槲皮素或人血浆中的三种主要槲皮素代谢物（槲皮素3-葡萄糖醛酸苷（Q3G）、槲皮素3'-硫酸盐（Q3'S）和异鼠李素）联合预处理，然后与苯并[a]芘（BaP）共同孵育，以研究这些化合物对BaP诱导的BC有害作用的影响。所有浓度为10μM的槲皮素代谢物均抑制BaP+BC诱导的细胞死亡。Q3'S、Q3G和异鼠李素还分别使BaP±BC诱导的DNA损伤显著降低64%、60%和24%。按相似顺序，这些化合物使BaP+BC诱导的细胞色素P450（CYP）1A1/1A2表达下调10%-50%。Q3G和Q3'S显著降低了BaP+BC诱导的细胞内活性氧的形成；然而，Q3G在减少Fe/NTA诱导的BC损失方面效果最佳。槲皮素代谢物的联合作用具有相加性。本研究表明，槲皮素代谢物至少部分地通过下调CYP1A1/1A2的表达来降低BaP诱导的β-胡萝卜素在A549细胞中的有害作用。
