Cong Qian, Borek Dominika, Otwinowski Zbyszek, Grishin Nick V
Department of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA.
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050, USA; Department of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA.
Cell Rep. 2015 Feb 17;10(6):910-919. doi: 10.1016/j.celrep.2015.01.026. Epub 2015 Feb 13.
Predicting phenotype from genotype represents the epitome of biological questions. Comparative genomics of appropriate model organisms holds the promise of making it possible. However, the high heterozygosity of many Eukaryotes currently prohibits assembling their genomes. Here, we report the 376 Mb genome sequence of Papilio glaucus (Pgl), the first sequenced genome from the Papilionidae family. We obtained the genome from a wild-caught specimen using a cost-effective strategy that overcomes the high (2%) heterozygosity problem. Comparative analyses suggest the molecular bases of various phenotypic traits, including terpene production in the Papilionidae-specific organ, osmeterium. Comparison of Pgl and Papilio canadensis transcriptomes reveals mutation hotspots (4% genes) associated with their divergence: four key circadian clock proteins are enriched in inter-species mutations and likely responsible for the difference in pupal diapause. Finally, the Pgl genome confirms Papilio appalachiensis as a hybrid of Pgl and Pca, but suggests it inherited 3/4 of its genes from Pca.
从基因型预测表型是生物学问题的核心。对合适的模式生物进行比较基因组学研究有望实现这一目标。然而，目前许多真核生物的高杂合性阻碍了其基因组的组装。在此，我们报告了北美大黄凤蝶（Papilio glaucus，简称Pgl）的376 Mb基因组序列，这是凤蝶科首个测序的基因组。我们采用一种经济高效的策略，从一只野生捕获的标本中获得了该基因组，该策略克服了高（2%）杂合性问题。比较分析揭示了各种表型特征的分子基础，包括凤蝶科特有的器官——臭角中的萜烯生成。Pgl和加拿大凤蝶（Papilio canadensis）转录组的比较揭示了与其分化相关的突变热点（4%的基因）：四种关键的生物钟蛋白在种间突变中富集，可能是蛹滞育差异的原因。最后，Pgl基因组证实了阿巴拉契亚凤蝶（Papilio appalachiensis）是Pgl和Pca的杂交种，但表明它从Pca继承了3/4的基因。
Zotero 插件
