Zheng Wenjing, Li Zhiqiang, Zhao Jiaming, Zhang Yanzhi, Wang Changhua, Lu Xiaochun, Sun Fuyu
The Crop Molecular Improving Laboratory, Liaoning Innovation Centre, Academy of Agriculture Sciences, Shenyang, China.
Liaoning Plant Protection Institute, Academy of Agriculture Sciences, Shenyang, China.
Pest Manag Sci. 2016 Feb;72(2):298-305. doi: 10.1002/ps.3992. Epub 2015 Mar 5.
The small brown planthopper (Laodelphax striatellus) is a widespread insect pest of rice in East Asia. Previous studies have shown the long-distance migrations undertaken by L. striatellus, but have not provided molecular evidence to support this.
Long-distance immigration has occurred in the north-east coastal rice-growing region of China. Using the specific-locus amplified fragment sequencing technique, sequence data for 2.7 Gb of an abruptly increased population and 13 L. striatellus local populations from a range of regions in China that have serious rice stripe disease were obtained. A total of 2572 single nucleotide polymorphisms (SNPs) and 37 indels were detected, and the genotypes of many polymorphism sites were heterozygous in every sample, which indicated that there were rich genetic differences among the populations, and that the migration of insect pests accelerated the gene flow and increased the heterozygosity of L. striatellus populations. The genetic distance and the polymorphism markers among different populations showed that the abruptly increased population in Liaoning Province is close to several populations from Jiangsu Province and Shandong Province.
The vector that caused rice stripe disease in the north-east of China was an immigrant population; however, the population may be formed from several groups from different areas, such as Jiangsu and Shandong provinces.
灰飞虱是东亚地区广泛分布的水稻害虫。以往研究表明灰飞虱存在远距离迁飞现象，但未提供分子证据支持。
中国东北沿海水稻种植区发生了远距离迁入。利用特异性位点扩增片段测序技术，获得了一个种群数量突然增加的2.7Gb序列数据以及来自中国水稻条纹叶枯病严重的多个地区的13个灰飞虱本地种群的序列数据。共检测到2572个单核苷酸多态性（SNP）和37个插入缺失，且每个样本中许多多态性位点的基因型为杂合型，这表明种群间存在丰富的遗传差异，害虫的迁飞加速了基因流动并增加了灰飞虱种群的杂合度。不同种群间的遗传距离和多态性标记显示，辽宁省种群数量突然增加的种群与江苏省和山东省的几个种群相近。
中国东北地区引起水稻条纹叶枯病的媒介是一个迁入种群；然而，该种群可能由来自不同地区（如江苏和山东）的几个群体组成。
