Du Xiao, Zhang Xiao, Bu Haidong, Zhang Ticao, Lao Yongchun, Dong Wenxuan
College of Horticulture, Shenyang Agricultural University, Shenyang, China.
Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang, China.
Front Plant Sci. 2019 Apr 9;10:443. doi: 10.3389/fpls.2019.00443. eCollection 2019.
Hawthorn is of high economic value owing to its medicinal properties and health benefits. is a member of the Rosaceae family; the genus has a complicated taxonomic history, and several theories on its origin have been proposed. In this study, 53 accessions from seven taxa native to China and accessions of exotic species (two from Europe and one from North America) were analyzed by specific locus amplified fragment sequencing (SLAF-seq). In total, 933,450 single-nucleotide polymorphisms were identified after filtering and used to investigate the species' genomic evolution. Phylogenetic trees derived from nuclear simple sequence repeats (SSRs) and SLAF-seq data showed the same topology, in which and formed a closely related cluster that was clearly separated from the cluster composed of , , var. , and . Phylogenetic and structure analysis indicated that the seven Chinese taxa had two separate speciation events. Plants that evolved the southwestern route shared the genepool with the European species, whereas plants along the northeastern route shared the genepool with the North American species. TreeMix genetic analysis revealed that may have a hybrid origin. This study provides valuable information on the origins of Chinese and suggests an evolutionary model for the main species that native to China.
山楂因其药用特性和对健康的益处而具有很高的经济价值。它是蔷薇科的一员；该属具有复杂的分类历史，并且已经提出了几种关于其起源的理论。在本研究中，通过特异性位点扩增片段测序（SLAF-seq）分析了来自中国本土七个山楂类群的53份种质以及外来山楂物种的种质（两份来自欧洲，一份来自北美）。经过筛选，共鉴定出933,450个单核苷酸多态性，并用于研究该物种的基因组进化。从核简单序列重复（SSR）和SLAF-seq数据推导的系统发育树显示出相同的拓扑结构，其中山楂和山楂形成了一个密切相关的聚类，该聚类与由山楂、山楂、山楂变种、山楂和山楂组成的聚类明显分开。系统发育和结构分析表明，七个中国山楂类群有两个独立的物种形成事件。沿着西南路线进化的植物与欧洲物种共享基因库，而沿着东北路线的植物与北美物种共享基因库。TreeMix遗传分析表明山楂可能有杂交起源。本研究为中国山楂的起源提供了有价值的信息，并提出了中国本土主要山楂物种的进化模型。
