Mawlood Shakhawan K, Alrowaithi Majid, Watson Nigel
Centre for Forensic Science, Department of Pure and Applied Chemistry (WestCHEM), University of Strathclyde, Royal College R 6.20, 204 George Street, G1 1XW, Glasgow, UK.
J Forensic Sci. 2015 May;60(3):686-9. doi: 10.1111/1556-4029.12704. Epub 2015 Feb 15.
This study compares two novel swabs (forensiX) with a standard cotton swab (EUROTUBE) for the collection of saliva stains on glass slide for STR analysis. ForensiX collection tubes are a standard cotton swab in an "active drying" tube, where swab sample is soon dried by its innovative tube surface of the wall. The other is forensiX Nylon Flocked Swab. The study is two phases: The first "phase" assesses swab types regarding to retrieve ability of saliva. The second "phase" compares the drying ability of each swab to assess how crime samples would fare when left in storage. The main result showed that "active drying" is effective to store swabbed sample. The forensiX swabs generally are effective for higher (twofold to fourfold) DNA yield compared to delta lab swab (around 750 pg and 250 pg from 0.5 μL of saliva), respectively. These findings demonstrate the importance of drying performance in the preservation of DNA and swab selection.
本研究比较了两种新型拭子（forensiX）与标准棉拭子（EUROTUBE）用于在载玻片上采集唾液污渍以进行STR分析的情况。ForensiX采集管是置于“主动干燥”管中的标准棉拭子，其拭子样本会通过创新的管壁表面迅速干燥。另一种是forensiX尼龙植绒拭子。该研究分为两个阶段：第一个“阶段”评估拭子类型在唾液获取能力方面的表现。第二个“阶段”比较每种拭子的干燥能力，以评估犯罪样本在储存时的情况。主要结果表明，“主动干燥”对于储存拭子样本是有效的。与delta lab拭子相比，forensiX拭子通常能产生更高（两倍至四倍）的DNA产量（分别从0.5μL唾液中获得约750 pg和250 pg）。这些发现证明了干燥性能在DNA保存和拭子选择中的重要性。
