Liu Jason Y
Thermo Fisher Scientific 180 Oyster Point Blvd., South San Francisco, CA, 94080.
J Forensic Sci. 2015 May;60(3):693-701. doi: 10.1111/1556-4029.12705. Epub 2015 Feb 15.
The PE-Swab direct STR amplification workflow was developed to process low-level "touch DNA" samples. In this workflow, a forensic sample is first collected on a 4-mm PE-Swab (a novel sample collection device); two 2-mm punches containing collected samples are then generated from the PE-Swab and directly amplified for STR typing. Compared to the conventional STR workflow, which involves DNA extraction, purification, and elution volume reduction, the PE-Swab direct STR amplification workflow does not require sample preparation and takes <60 sec before a touch sample is ready for STR amplification. Because there is no DNA loss due to sample preparation, the PE-Swab workflow is more sensitive than the conventional STR workflow. The average peak height per sample obtained by the PE-swab workflow is 3 times higher than that from the conventional workflow with both low-level single source and two-contributor mixture samples tested in this study.
PE拭子直接STR扩增工作流程是为处理低水平“接触DNA”样本而开发的。在此工作流程中，首先在4毫米的PE拭子（一种新型样本采集装置）上采集法医样本；然后从PE拭子上切下两个包含采集样本的2毫米冲片，并直接进行STR分型扩增。与传统的STR工作流程（包括DNA提取、纯化和洗脱体积减少）相比，PE拭子直接STR扩增工作流程不需要样本制备，在接触样本准备好进行STR扩增之前所需时间不到60秒。由于样本制备过程中不会造成DNA损失，PE拭子工作流程比传统STR工作流程更灵敏。在本研究中测试的低水平单源和双供体混合样本中，PE拭子工作流程获得的每个样本的平均峰高比传统工作流程高3倍。
