Tawil Mirna, Bekdash Amira, Mroueh Mohammad, Daher Costantine F, Abi-Habib Ralph J
Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, Beirut, Lebanon E-mail :
Asian Pac J Cancer Prev. 2015;16(2):761-7. doi: 10.7314/apjcp.2015.16.2.761.
In this study, we used Daucus carota oil extract (DCOE) to target acute myeloid leukemia (AML) cells. All the AML cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. Analysis of mechanism of cell death showed an increase in cells positive for annexinV and for active caspases, indicating that DCOE induces apoptotic cell death in AML. Inhibition of the MAPK pathway decreased sensitivity of AML cells to DCOE, indicating that cytotoxicity may be dependent on its activity. In conclusion, DCOE induces selective apoptosis in AML cells, possibly through a MAPK-dependent mechanism.
在本研究中，我们使用胡萝卜籽油提取物（DCOE）靶向急性髓系白血病（AML）细胞。所有测试的AML细胞系对该提取物敏感，而外周血单个核细胞则不敏感。细胞死亡机制分析显示，膜联蛋白V和活性半胱天冬酶阳性细胞增加，表明DCOE诱导AML细胞凋亡。抑制丝裂原活化蛋白激酶（MAPK）途径可降低AML细胞对DCOE的敏感性，表明细胞毒性可能依赖于其活性。总之，DCOE可能通过MAPK依赖机制诱导AML细胞选择性凋亡。
