Bugan Ilknur, Altun Seyhan
Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey E-mail :
Asian Pac J Cancer Prev. 2015;16(2):831-6. doi: 10.7314/apjcp.2015.16.2.831.
Tumor fluid accumulation occurs in both human cancer and experimental tumor models. Solid tumors show a tendency to tumor fluid accumulation because of their anatomical and physiological features and this may be influenced by molecular factors. Fluid accumulation in the peri-tumor area also occurs in the Dunning model of rat prostate cancer as the tumor grows. In this study, the effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell's migration and tumor fluid's toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index. In this research, tumor fluids were obtained from rats injected with 25105 MAT- LyLu cells and treated with saline solution, and 200 nM tetrodotoxin (TTX), highly specific sodium channel blocker was used. Sterilized tumor fluids were added to medium of MAT-LyLu cells with the proportion of 20% in vitro. Consequently, it was demonstrated that Dunning rat prostate tumor fluid significantly inhibited proliferation (up to 50%), mitotic index, and labeling index of MAT-LyLu cells (up to 75%) (p<0.05) but stimulated the motility of the cells in vitro.
肿瘤积液在人类癌症和实验性肿瘤模型中均会出现。实体瘤由于其解剖学和生理学特征呈现出肿瘤积液的倾向，并且这可能受分子因素影响。在大鼠前列腺癌的邓宁模型中，随着肿瘤生长，肿瘤周围区域也会出现积液。在本研究中，通过检测细胞迁移、肿瘤液毒性以及细胞增殖、有丝分裂指数和标记指数等动力学参数，研究了从携带邓宁前列腺肿瘤的哥本哈根大鼠获取的肿瘤液对高转移性MAT-LyLu细胞系的影响。在本研究中，肿瘤液取自注射25×10⁵个MAT-LyLu细胞并用盐溶液处理的大鼠，同时使用了200 nM河豚毒素（TTX），一种高度特异性的钠通道阻滞剂。将经过灭菌的肿瘤液以20%的比例添加到体外培养的MAT-LyLu细胞培养基中。结果表明，邓宁大鼠前列腺肿瘤液显著抑制MAT-LyLu细胞的增殖（高达50%）、有丝分裂指数和标记指数（高达75%）（p<0.05），但在体外刺激了细胞的运动能力。
