Fraser S P, Ding Y, Liu A, Foster C S, Djamgoz M B
Department of Biology, Neurobiology Group, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK.
Cell Tissue Res. 1999 Mar;295(3):505-12. doi: 10.1007/s004410051256.
Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyLu and the AT-2 cell line for 24 h with the Na+ channel blocker tetrodotoxin (TTX) at 6 microM altered the morphology only of the MAT-LyLu cell line. TTX produced significant decreases in: (a) cell process length and (b) field diameter, and increases in (c) cell body diameter and (d) process thickness. Importantly, 6 microM TTX had no significant effects on proliferation rates or cellular toxicity. The results suggest that Na+ channel activity plays a significant role in determining the morphological development of MAT-LyLu cells in such a way as to enhance their metastatic potential.
电压门控性钠离子通道在源自啮齿动物前列腺癌邓宁模型的高转移性MAT-LyLu细胞中表达，但在低转移性AT-2细胞中不表达。我们研究了这些通道可能参与细胞形态发育的情况。用6微摩尔的钠离子通道阻滞剂河豚毒素（TTX）将MAT-LyLu和AT-2细胞系孵育24小时，仅改变了MAT-LyLu细胞系的形态。TTX导致以下各项显著降低：（a）细胞突起长度和（b）视野直径，以及（c）细胞体直径和（d）突起厚度增加。重要的是，6微摩尔的TTX对增殖率或细胞毒性没有显著影响。结果表明，钠离子通道活性在决定MAT-LyLu细胞的形态发育方面起着重要作用，从而增强其转移潜能。
