阿塞拜疆慢性乙型肝炎感染患者中乙肝病毒基因型的分布
Distribution of hepatitis B virus genotypes in azerbaijani patients with chronic hepatitis B infection.
作者信息
Bokharaei-Salim Farah, Keyvani Hossein, Monavari Seyed Hamidreza, Esghaei Maryam, Fakhim Shahin, Ataei Pirkooh Angila, Behnava Bita
机构信息
Department of Virology, Iran University of Medical Sciences, Tehran, IR Iran.
Department of Civil Engineering, Faculty of Engineering, Payame Noor University, Karaj, IR Iran.
出版信息
Hepat Mon. 2014 Dec 9;14(12):e25105. doi: 10.5812/hepatmon.25105. eCollection 2014 Dec.
BACKGROUND
Hepatitis B virus (HBV) has been classified into ten genotypes (A-J) based on genome sequence divergence, which is very important for etiological and clinical investigations. HBV genotypes have distinct geographical distributions worldwide.
OBJECTIVES
The aim of this study was to investigate the distribution of HBV genotypes among Azerbaijani patients with chronic hepatitis B, came from the Republic of Azerbaijan country to Iran to receive medical care.
PATIENTS AND METHODS
One hundred and three patients with chronic HBV infection, referred to hospitals related to Iran University of Medical Sciences and Tehran Hepatitis Center from August 2011 to July 2014, were enrolled in this cross sectional study. About 3-milliliter of peripheral blood was taken from each patient. After viral DNA extraction, HBV genotypes were tested using the INNO-LiPA™ HBV kit (Innogenetics, Ghent, Belgium). HBV genotyping was confirmed using sequencing of hepatitis B surface antigen (HBsAg) and polymerase (pol) regions of HBV.
RESULTS
The mean age of patients was 35.9 ± 11.7 years (19-66). Of 103 patients, 72 (69.9%) were male. In the present study, the predominant HBV genotype was D (93.2%) followed by genotype A (5.8%) and concurrent infection with A and D genotypes (0.97%).
CONCLUSIONS
The main and frequent HBV genotype among Azerbaijani patients with chronic hepatitis B virus infection was genotype D followed by genotype A.
背景
基于基因组序列差异，乙型肝炎病毒（HBV）已被分为十种基因型（A - J），这对病因学和临床研究非常重要。HBV基因型在全球具有不同的地理分布。
目的
本研究旨在调查从阿塞拜疆共和国前往伊朗接受医疗护理的慢性乙型肝炎阿塞拜疆患者中HBV基因型的分布情况。
患者与方法
2011年8月至2014年7月转诊至与伊朗医科大学和德黑兰肝炎中心相关医院的103例慢性HBV感染患者纳入本横断面研究。从每位患者采集约3毫升外周血。病毒DNA提取后，使用INNO - LiPA™ HBV试剂盒（Innogenetics，比利时根特）检测HBV基因型。通过对HBV的乙型肝炎表面抗原（HBsAg）和聚合酶（pol）区域进行测序来确认HBV基因分型。
结果
患者的平均年龄为35.9±11.7岁（19 - 66岁）。103例患者中，72例（69.9%）为男性。在本研究中，主要的HBV基因型是D型（93.2%），其次是A型（5.8%）以及A和D基因型的合并感染（0.97%）。
结论
慢性乙型肝炎病毒感染的阿塞拜疆患者中主要且常见的HBV基因型是D型，其次是A型。
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