Rao Pss, Sari Y
Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, USA.
J Addict Res Ther. 2014;5. doi: 10.4172/2155-6105.1000183.
To evaluate the effectiveness of ceftriaxone treatment in attenuating relapse-like ethanol drinking behavior in male P rats following 14-weeks of continuous ethanol consumption.
After 14-weeks of continuous access to free choice of 15% and 30% ethanol, male P rats were deprived of ethanol for two weeks. On the last five days of abstinence period, P rats were treated, once a day, with either saline or ceftriaxone (50 or 200 mg/kg; i.p.). This was followed by re-exposure to ethanol for the next 10 days to simulate the relapse-like ethanol drinking behavior.
Ceftriaxone treatment (during abstinence) reduced ethanol intake upon re-exposure to ethanol, compared to the saline treated P rats. This statistically significant reduction in ethanol consumption in P rats following treatment with ceftriaxone (200 mg/kg/day) was observed from Day 2 to Day 9. Similarly, water consumption in P rats treated with ceftriaxone was significantly higher than the saline treated group between Day 2 and Day 7. Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.
We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model. This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.
评估头孢曲松治疗对连续摄入乙醇14周的雄性P大鼠复发性乙醇饮用行为的缓解效果。
在连续14周自由选择15%和30%乙醇后，雄性P大鼠被剥夺乙醇两周。在禁欲期的最后五天，P大鼠每天接受一次生理盐水或头孢曲松（50或200mg/kg；腹腔注射）治疗。随后在接下来的10天内再次接触乙醇，以模拟复发性乙醇饮用行为。
与生理盐水处理的P大鼠相比，头孢曲松治疗（禁欲期间）在再次接触乙醇时减少了乙醇摄入量。在用头孢曲松（200mg/kg/天）治疗后的P大鼠中，从第2天到第9天观察到乙醇消耗的这种统计学显著减少。同样，在第2天到第7天之间，用头孢曲松治疗的P大鼠的水消耗量明显高于生理盐水处理组。重要的是，与生理盐水处理组相比，两种剂量的头孢曲松治疗均未引起体重的任何显著变化。
我们在此报告，已发现高剂量的头孢曲松在慢性乙醇摄入模型中对缓解复发性乙醇饮用行为有效。这与我们实验室先前在可卡因动物模型中的数据一致，该数据表明只有高剂量的头孢曲松在减轻可卡因复发方面有效。
