Puymirat J, Luo M, Dussault J H
Laboratoire d'Ontogénèse et de génètique moléculaire, CHU Laval, Québec, Canada.
Neuroscience. 1989;30(2):443-9. doi: 10.1016/0306-4522(89)90264-9.
By means of a monoclonal antibody against the rat liver L-triiodothyronine nuclear receptor and a polyclonal anti-tyrosine hydroxylase serum, it has been possible to demonstrate thyroid hormone nuclear receptors in immunoreactive tyrosine hydroxylase cell nuclei in fetal rat hypothalamic cultures. After 8 days in vitro, the ratio of tyrosine hydroxylase cells that were immunoreactive for the thyroid hormone receptor to those not stained for this receptor (64% to 36% respectively) remains unchanged despite an increase in the number of tyrosine hydroxylase-positive cells with time (from day 8 to day 21) in culture. The presence of thyroid hormone nuclear receptor in dopaminergic neurons is correlated with a morphological effect of L-triiodothyronine in this neuronal population. Our results demonstrate, for the first time, the presence of triiodothyronine nuclear receptors in fetal rat dopaminergic neurons and the existence of a cellular heterogeneity in the distribution of the thyroid hormone receptor. The presence of these receptors in fetal hypothalamic dopaminergic neurons suggests that some effects of L-triiodothyronine on the maturation of DA neurons may result from a direct effect of this hormone through an interaction with its specific nuclear receptors.
借助抗大鼠肝脏L-三碘甲状腺原氨酸核受体的单克隆抗体和抗酪氨酸羟化酶多克隆血清，已能够在胎鼠下丘脑培养物中免疫反应性酪氨酸羟化酶细胞核内证实甲状腺激素核受体的存在。体外培养8天后，对甲状腺激素受体呈免疫反应性的酪氨酸羟化酶细胞与未被该受体染色的细胞之比（分别为64%和36%）保持不变，尽管培养过程中（从第8天到第21天）酪氨酸羟化酶阳性细胞数量随时间增加。多巴胺能神经元中甲状腺激素核受体的存在与L-三碘甲状腺原氨酸对该神经元群体的形态学效应相关。我们的结果首次证明了胎鼠多巴胺能神经元中存在三碘甲状腺原氨酸核受体，以及甲状腺激素受体分布存在细胞异质性。胎鼠下丘脑多巴胺能神经元中这些受体的存在表明，L-三碘甲状腺原氨酸对多巴胺能神经元成熟的某些影响可能是该激素通过与其特异性核受体相互作用产生的直接效应。
