Lezoualc'h F, Hassan A, Abdel-Tawab H, Puymirat J, Demeneix B A
Laboratoire de Physiologie Générale et Comparée, URA 90 CNRS, Muséum National d'Histoire Naturelle, Paris, France.
Neurosci Lett. 1994 Oct 24;180(2):197-202. doi: 10.1016/0304-3940(94)90520-7.
Qualitative immunocytochemical and quantitative autoradiographic approaches were used to examine the presence of thyroid hormone receptor (TR) proteins in embryonic chick hypothalamic cultures and the effect of T3 on their expression. We used antibodies raised against rat TRs having first verified that they recognised nuclear T3 binding proteins in extracts from chick brains. TRs were expressed in embryonic hypothalamic neurons from 6 day-old chick embryos maintained 4 days in vitro, and their expression was up-regulated by T3. This demonstration of TRs in embryonic hypothalamic neurons suggests that a physiological mechanism for early regulation of the hypothalamic neuropeptide, thyrotropin releasing hormone, is installed prior to the onset of thyroid function.
采用定性免疫细胞化学和定量放射自显影方法，检测胚胎鸡下丘脑培养物中甲状腺激素受体（TR）蛋白的存在情况以及T3对其表达的影响。我们使用了针对大鼠TRs产生的抗体，首先验证了它们能识别鸡脑提取物中的核T3结合蛋白。TRs在体外培养4天的6日龄鸡胚的胚胎下丘脑神经元中表达，并且其表达受到T3的上调。胚胎下丘脑神经元中TRs的这一证明表明，下丘脑神经肽促甲状腺激素释放激素的早期调节生理机制在甲状腺功能开始之前就已建立。
