West Christopher W, Bull James N, Hudson Alex S, Cobb Steven L, Verlet Jan R R
Department of Chemistry, Durham University , Durham, DH1 3LE, United Kingdom.
J Phys Chem B. 2015 Mar 12;119(10):3982-7. doi: 10.1021/acs.jpcb.5b01432. Epub 2015 Feb 25.
A combined frequency-, angle-, and time-resolved photoelectron spectroscopy study is used to unravel the excited state dynamics following UV excitation of the isolated anionic chromophore of the green fluorescent protein (GFP). The optically bright S3 state, which is populated for hv > 3.7 eV, is shown to decay predominantly by internal conversion to the S2 state that in turn autodetaches to the neutral ground state. For hv > 4.1 eV, a new and favorable autodetachment channel from the S2 state becomes available, which leads to the formation of the neutral in an excited state. The results indicate that the UV excited state dynamics of the GFP chromophore involve a number of strongly coupled excited states.
一项结合频率、角度和时间分辨光电子能谱的研究被用于揭示绿色荧光蛋白（GFP）孤立阴离子发色团在紫外激发后的激发态动力学。对于光子能量（hv）> 3.7 eV时所填充的光学明亮的S3态，显示其主要通过内转换衰减至S2态，而S2态继而自解离至中性基态。对于hv > 4.1 eV，从S2态出现了一个新的且有利的自解离通道，这导致在激发态形成中性物质。结果表明，GFP发色团的紫外激发态动力学涉及多个强耦合的激发态。
