Clarke Lorelei L, Ballweber Lora R, Allen Kelly, Little Susan E, Lappin Michael R
Department of Clinical Sciences, Colorado State University.
J S Afr Vet Assoc. 2014 Sep 11;85(1):996. doi: 10.4102/jsava.v85i1.996.
Ticks, sera and ethylenediaminetetraacetic acid (EDTA) blood were collected from dogs evaluated at the Amakom Veterinary Clinic in Kumasi, Ghana. Sera were evaluated for Dirofilaria immitis antigen and antibodies against Borrelia burgdorferi, Anaplasma phagocytophilum and Ehrlichia canis. Conventional polymerase chain reaction assays designed to amplify the deoxyribonucleic acid (DNA) of Ehrlichia spp. or Anaplasma spp. or Neorickettsia spp. or Wolbachia spp., Babesia spp., Rickettsia spp., Hepatozoon spp., Bartonella spp. and the haemoplasmas were performed on DNA extracted from EDTA blood and all positive amplicons were sequenced. This small survey shows that the following vector-borne pathogens are present in urban Ghanian dogs: Ehrlichia canis, Hepatozoon canis,Dirofilaria immitis and Anaplasma platys. Bartonella henselae was isolated from ticks but not from the dogs.
蜱虫、血清和乙二胺四乙酸（EDTA）抗凝血采自于在加纳库马西阿马科姆兽医诊所接受评估的犬只。检测血清中的犬恶丝虫抗原以及针对伯氏疏螺旋体、嗜吞噬无形体和犬埃立克体的抗体。设计常规聚合酶链反应检测法，用于扩增埃立克体属、无形体属、新立克次体属、沃尔巴克氏体属、巴贝斯虫属、立克次体属、肝簇虫属、巴尔通体属和血巴尔通体属的脱氧核糖核酸（DNA），对从EDTA抗凝血中提取的DNA进行检测，并对所有阳性扩增子进行测序。这项小型调查显示，加纳城市犬只感染了以下媒介传播病原体：犬埃立克体、犬肝簇虫、犬恶丝虫和血小板无形体。从蜱虫中分离出了汉赛巴尔通体，但未从犬只中分离出。
