铁硫蛋白的高级顺磁共振波谱学：电子核双共振（ENDOR）和电子自旋回波包络调制（ESEEM）。
Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM).
作者信息
Cutsail George E, Telser Joshua, Hoffman Brian M
机构信息
Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Department of Biological, Chemical and Physical Sciences, Roosevelt University, Chicago, IL 60605, USA.
出版信息
Biochim Biophys Acta. 2015 Jun;1853(6):1370-94. doi: 10.1016/j.bbamcr.2015.01.025. Epub 2015 Feb 14.
Abstract
The advanced electron paramagnetic resonance (EPR) techniques, electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) spectroscopies, provide unique insights into the structure, coordination chemistry, and biochemical mechanism of nature's widely distributed iron-sulfur cluster (FeS) proteins. This review describes the ENDOR and ESEEM techniques and then provides a series of case studies on their application to a wide variety of FeS proteins including ferredoxins, nitrogenase, and radical SAM enzymes. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
摘要
先进的电子顺磁共振（EPR）技术，即电子-核双共振（ENDOR）和电子自旋回波包络调制（ESEEM）光谱学，为深入了解自然界广泛分布的铁硫簇（FeS）蛋白的结构、配位化学和生化机制提供了独特视角。本综述介绍了ENDOR和ESEEM技术，随后提供了一系列关于它们应用于多种FeS蛋白的案例研究，这些蛋白包括铁氧化还原蛋白、固氮酶和自由基S-腺苷甲硫氨酸酶。本文是名为《Fe/S蛋白：分析、结构、功能、生物合成及疾病》的特刊的一部分。
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