Gu Xianbin, Chen Yahua, Gao Zhihong, Qiao Yushan, Wang Xiuyun
College of Horticulture, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China; College of Life Sciences, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
College of Life Sciences, Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, People's Republic of China.
Plant Physiol Biochem. 2015 Apr;89:31-43. doi: 10.1016/j.plaphy.2015.02.004. Epub 2015 Feb 11.
Dehydration-responsive element-binding (DREB) transcription factors play critical roles in plant stress responses and signal transduction. To further understand how DREB regulates genes expression to promote cold-hardiness, Illumina/Solexa sequencing technology was used to compare the transcriptomes of non-transgenic and rd29A:RdreB1BI transgenic strawberry plants exposed to low temperatures. Approximately 3.5 million sequence tags were obtained from non-transgenic (NT) and transgenic (T) strawberry untreated (C) or low-temperature treated (LT) leaf samples. Over 1000 genes were differentially expressed between the NT-C and T-C plants, and also the NT-C and NT-LT, as well as the T-C and T-LT plants. Analysis of the genes up-regulated following low-temperature treatment revealed that the majority are linked to metabolism, biosynthesis, transcription and signal transduction. Uniquely up-regulated transcription factors as well as anthocyanin biosynthetic pathway genes are discussed. Accumulation of anthocyanin in the stolon and the base of the petiole differed between non-treated NT and T plants, and this correlated with gene expression patterns. The differentially expressed genes that encode transcription factors and anthocyanin enzymes may contribute to the cold hardiness of RdreB1BI transgenic strawberry. The transcriptome data provide a valuable resource for further studies of strawberry growth and development and DREB-mediated gene regulation under low-temperature stress.
脱水响应元件结合（DREB）转录因子在植物应激反应和信号转导中发挥着关键作用。为了进一步了解DREB如何调节基因表达以提高抗寒性，利用Illumina/Solexa测序技术比较了未转基因和rd29A:RdreB1BI转基因草莓植株在低温处理下的转录组。从未经处理（C）或低温处理（LT）的非转基因（NT）和转基因（T）草莓叶片样本中获得了约350万个序列标签。在NT-C和T-C植株之间、NT-C和NT-LT之间以及T-C和T-LT之间，有超过1000个基因存在差异表达。对低温处理后上调的基因分析表明，大多数基因与代谢、生物合成、转录和信号转导有关。文中讨论了独特上调的转录因子以及花青素生物合成途径基因。未处理的NT和T植株在匍匐茎和叶柄基部的花青素积累存在差异，这与基因表达模式相关。编码转录因子和花青素酶的差异表达基因可能有助于RdreB1BI转基因草莓的抗寒性。转录组数据为进一步研究草莓生长发育以及低温胁迫下DREB介导的基因调控提供了宝贵资源。
