Farha A K, Dhanya S R, Mangalam S Nair, Remani P
a Division of Cancer Research, Regional Cancer Centre , Thiruvananthapuram , Kerala , India.
b CSIR-National Institute for Interdisciplinary Science and Technology , Thiruvananthapuram , Kerala , India.
Nat Prod Res. 2015;29(24):2341-5. doi: 10.1080/14786419.2015.1012165. Epub 2015 Feb 17.
In this study, we focused on the in vitro anti-metastatic effects of deoxyelephantopin (DOE), a sesquiterpene lactone from Elephantopus scaber on lung cancer A549 cells. DOE significantly decreased the metastatic potential of A549 cells as demonstrated by transwell invasion and migration assay. DOE inhibited the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, urokinase-type plasminogen activator and urokinase-type plasminogen activator receptor at transcript level. Tissue inhibitors of metalloproteinase-2 (TIMP-2) mRNA levels was up-regulated in A549 tumour cells without any change in TIMP-1 expression after DOE treatment. DOE inhibited the protein levels of p-ERK1/2 and p-Akt in A549 cells but it activated p-JNK, p-p38 protein expression. NF-κB and IκBα expressions were down-regulated in DOE-treated cells. All these results demonstrated that DOE has shown anti-metastatic activity against A549 tumour cells.
在本研究中，我们聚焦于去氧地胆草素（DOE）的体外抗转移作用，DOE是一种来源于地胆草的倍半萜内酯，作用于肺癌A549细胞。经Transwell侵袭和迁移试验表明，DOE显著降低了A549细胞的转移潜能。DOE在转录水平抑制基质金属蛋白酶-2（MMP-2）、MMP-9、尿激酶型纤溶酶原激活剂和尿激酶型纤溶酶原激活剂受体的表达。在DOE处理后，金属蛋白酶组织抑制剂-2（TIMP-2）mRNA水平在A549肿瘤细胞中上调，而TIMP-1表达无任何变化。DOE抑制A549细胞中p-ERK1/2和p-Akt的蛋白水平，但激活p-JNK、p-p38蛋白表达。在DOE处理的细胞中，NF-κB和IκBα表达下调。所有这些结果表明，DOE对A549肿瘤细胞具有抗转移活性。
