Tran Nhiem, Kelley Michael N, Tran Phong A, Garcia Dioscaris R, Jarrell John D, Hayda Roman A, Born Christopher T
Department of Orthopaedic Surgery, Alpert Medical School, Brown University, Providence, RI, USA; Weiss Center for Orthopaedic Trauma Research, Rhode Island Hospital, Providence, RI, USA.
Department of Orthopaedic Surgery, Alpert Medical School, Brown University, Providence, RI, USA; BioIntraface Inc., North Kingstown, RI, USA.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:201-209. doi: 10.1016/j.msec.2014.12.072. Epub 2014 Dec 23.
Bacterial infection remains one of the most serious issues affecting the successful installation and retention of orthopedic implants. Many bacteria develop resistance to current antibiotics, which complicates or prevents traditional antibiotic-dependent eradication therapy. In this study, a hybrid coating of titanium dioxide and polydimethylsiloxane (PDMS) was synthesized to regulate the release of silver. The coatings were benefited from the antimicrobial activity of silver ion, the biocompatibility of titanium dioxide, and the flexibility of the polymer. Three studied silver doped coatings with different titanium dioxide-PDMS ratios effectively inhibited the attachment and growth of Staphylococcus aureus and Staphylococcus epidermidis in a dose-dependent manner. The coatings were successfully applied on the discs of polyether ether ketone (PEEK), a common spinal implant material and antibacterial property of these coatings was assessed via Kirby Bauer assay. More importantly, these selected coatings completely inhibited biofilm formation. The release study demonstrated that the release rate of silver from the coating depended on doping levels and also the ratios of titanium dioxide and PDMS. This result is crucial for designing coatings with desired silver release rate on PEEK materials for antimicrobial applications.
细菌感染仍然是影响骨科植入物成功植入和留存的最严重问题之一。许多细菌对当前的抗生素产生耐药性，这使得传统的依赖抗生素的根除疗法变得复杂或无法进行。在本研究中，合成了一种二氧化钛和聚二甲基硅氧烷（PDMS）的混合涂层以调节银的释放。这些涂层受益于银离子的抗菌活性、二氧化钛的生物相容性以及聚合物的柔韧性。三种研究的不同二氧化钛 - PDMS比例的银掺杂涂层以剂量依赖的方式有效抑制了金黄色葡萄球菌和表皮葡萄球菌的附着和生长。这些涂层成功应用于聚醚醚酮（PEEK）圆盘上，PEEK是一种常见的脊柱植入材料，并通过 Kirby Bauer试验评估了这些涂层的抗菌性能。更重要的是，这些选定的涂层完全抑制了生物膜的形成。释放研究表明，涂层中银的释放速率取决于掺杂水平以及二氧化钛和PDMS的比例。这一结果对于在用于抗菌应用的PEEK材料上设计具有所需银释放速率的涂层至关重要。
