Banerjee Pradipta, Madhu S, Chandra Babu N K, Shanthi C
School of Bio Science and Technology, Vellore Institute of Technology University, Vellore 632014, Tamil Nadu, India.
Tannery Division, CSIR-Central Leather Research Institute, Chennai 600 020, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:338-347. doi: 10.1016/j.msec.2015.01.027. Epub 2015 Jan 8.
Hydroxyapatite (HA) ceramics serve as an alternative to autogenous-free bone grafting by virtue of their excellent biocompatibility. However, chemically synthesized HA lacks the strong load-bearing capacity as required by bone. The bio-mimetic growth of HA crystals on collagen surface provides a feasible solution for synthesizing bone substitutes with the desired properties. This study deals with the utilization of the collagen hydrolysate recovered from leather waste as a substrate for promoting HA crystal growth. Bio-mimetic growth of HA was induced by subjecting the hydrolysate to various mineralization conditions. Parameters that would have a direct effect on crystal growth were varied to determine the optimal conditions necessary. Maximum mineralization was achieved with a combination of 10mM of CaCl2, 5mM of Na2HPO4, 100mM of NaCl and 0.575% glutaraldehyde at a pH of 7.4. The metal-protein interactions leading to formation of HA were identified through Fourier-transform infrared (FTIR) spectroscopy and x-ray diffraction (XRD) studies. The crystal dimensions were determined to be in the nanoscale range by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The size and crystallinity of bio-mimetically grown HA indicate that hydrolysate from leather waste can be used as an ideal alternative substrate for bone growth.
羟基磷灰石（HA）陶瓷凭借其出色的生物相容性，成为自体骨移植的替代物。然而，化学合成的HA缺乏骨骼所需的强大承重能力。在胶原蛋白表面仿生生长HA晶体为合成具有所需特性的骨替代物提供了一种可行的解决方案。本研究探讨了利用从皮革废料中回收的胶原蛋白水解物作为促进HA晶体生长的底物。通过使水解物处于各种矿化条件下，诱导HA的仿生生长。改变对晶体生长有直接影响的参数，以确定所需的最佳条件。在pH值为7.4时，将10mM氯化钙、5mM磷酸氢二钠、100mM氯化钠和0.575%戊二醛组合使用可实现最大矿化。通过傅里叶变换红外（FTIR）光谱和X射线衍射（XRD）研究确定了导致HA形成的金属-蛋白质相互作用。通过原子力显微镜（AFM）和扫描电子显微镜（SEM）确定晶体尺寸处于纳米级范围。仿生生长的HA的尺寸和结晶度表明，皮革废料中的水解物可作为骨骼生长的理想替代底物。
