Hu Ying, Heo Cheol Ho, Kim Gyoungmi, Jun Eun Jin, Yin Jun, Kim Hwan Myung, Yoon Juyoung
†Department of Chemistry and Nano Science, Ewha Womans University, Global Top5 Research Program, Seoul 120-750, Korea.
‡Department of Energy Systems Research, Ajou University, Suwon, Gyeonggi-do 443-749, Korea.
Anal Chem. 2015 Mar 17;87(6):3308-13. doi: 10.1021/ac504372w. Epub 2015 Mar 2.
Glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are three major biothiols, which play key roles in various biological systems. Accordingly, the development of imaging probes has been actively studied. We report a new pyrene derivative 1, which showed large fluorescence quenching with Cu(2+) at pH 7.4. The ensemble 1-Cu(2+) was applied to detect biothiols. Among the various amino acids, GSH, Cys, and Hcy induced distinct turn-on fluorescence changes. The 1-Cu(2+) ensemble was further applied for GSH detection in living cells.
谷胱甘肽（GSH）、半胱氨酸（Cys）和同型半胱氨酸（Hcy）是三种主要的生物硫醇，它们在各种生物系统中发挥着关键作用。因此，成像探针的开发一直受到积极研究。我们报道了一种新的芘衍生物1，它在pH 7.4时与Cu(2+)表现出强烈的荧光猝灭。1-Cu(2+)组合被用于检测生物硫醇。在各种氨基酸中，GSH、Cys和Hcy引起了明显的荧光开启变化。1-Cu(2+)组合进一步用于活细胞中GSH的检测。
