Chesselet M F, Robbins E
Department of Pharmacology, Medical College of Pennsylvania, EPPI, Philadelphia 19129.
Brain Res. 1989 Jul 17;492(1-2):237-44. doi: 10.1016/0006-8993(89)90906-2.
Two types of labelled cells are detected in sections of rat and mouse striata processed for in situ hybridization histochemistry with 35S-radiolabelled RNA probes complementary to the messenger RNA (mRNA) encoding glutamic acid decarboxylase (GAD), the synthesis enzyme for gamma-aminobutyric acid (GABA): numerous lightly, and fewer very densely labelled neurons. In order to determine whether the densely labelled cells correspond to the striatal somatostatinergic neurons with which they share morphological characteristics, the presence of GAD mRNA was examined in brain sections processed successively for dihydronicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry, a marker of striatal somatostatinergic neurons, and in situ hybridization histochemistry. In addition, the distribution of GABAergic interneurons was analyzed with regard to striatal compartments (striosomes) indicated by patches of dense opiate binding sites. The results show that NADPH diaphorase activity and GAD mRNA do not co-exist in striatal neurons. Furthermore, in contrast to the somatostatinergic neurons which are almost exclusively located in the extrastriosomal matrix, densely labelled GAD cells were present both in the striosomes and the matrix, further suggesting that GABAergic and somatostatinergic neurons form two distinct interneuronal systems in the striatum of rats and mice.
在用与编码γ-氨基丁酸（GABA）合成酶谷氨酸脱羧酶（GAD）的信使核糖核酸（mRNA）互补的35S放射性标记RNA探针进行原位杂交组织化学处理的大鼠和小鼠纹状体切片中，检测到两种类型的标记细胞：许多轻度标记和较少的高度密集标记的神经元。为了确定高度密集标记的细胞是否与具有相同形态特征的纹状体生长抑素能神经元相对应，在先后进行二氢烟酰胺腺嘌呤二核苷酸磷酸（NADPH）黄递酶组织化学（纹状体生长抑素能神经元的一种标记）和原位杂交组织化学处理的脑切片中检测GAD mRNA的存在。此外，根据由密集阿片类结合位点斑块指示的纹状体区室（纹状体小体）分析了GABA能中间神经元的分布。结果表明，NADPH黄递酶活性和GAD mRNA在纹状体神经元中不存在共存。此外，与几乎仅位于纹状体小体外基质中的生长抑素能神经元相反，高度密集标记的GAD细胞在纹状体小体和基质中均存在，这进一步表明GABA能和生长抑素能神经元在大鼠和小鼠纹状体中形成两个不同的中间神经元系统。
